The murine PSE/TATA-dependent transcriptome: Evidence of functional homologies with its human counterpart

Maria Jessica Bruzzone, Paola Gavazzo, Sara Massone, Carolina Balbi, Federico Villa, Anastasia Conti, Giorgio Dieci, Ranieri Cancedda, Aldo Pagano

Research output: Contribution to journalArticlepeer-review

Abstract

A series of recent studies demonstrated an unexpectedly high frequency of intronic RNA polymerase (pol) III transcription units spread throughout the human genome. The investigation of a subset of these transcripts revealed their tissue/cell-specific transcription together with the involvement in relevant physiopathological pathways. Despite this evidence, these transcripts did not seem to have murine orthologs, based on their nucleotide sequence, resulting in a limitation of the experimental approaches aimed to study their function. In this work, we have extended our investigation to the murine genome identifying 121 pairs of mouse/human transcripts displaying syntenic subchromosomal localization. The analysis in silico of this set of putative noncoding (nc)RNAs suggest their association with alternative splicing as suggested by recent experimental evidence. The investigation of one of these pairs taken as experimental model in mouse hippocampal neurons provided evidence of a human/mouse functional homology that does not depend on underlying sequence conservation. In this light, the collection of transcriptional units here reported can be considered as a novel source for the identification and the study of novel regulatory elements involved in relevant biological processes.

Original languageEnglish
Pages (from-to)14813-14827
Number of pages15
JournalInternational Journal of Molecular Sciences
Volume13
Issue number11
DOIs
Publication statusPublished - 2012

Keywords

  • Alternative splicing
  • Non-coding RNA
  • Potassium channel interacting protein (KCNIP4)
  • RNA polymerase III

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Medicine(all)

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