The myenteric plexus of the esophagus is abnormal in an experimental congenital diaphragmatic hernia model

L. Martinez, F. Pederiva, W. Martinez-Calonge, R. Aras-Lopez, J. A. Tovar

Research output: Contribution to journalArticlepeer-review


Background/aim: Infants surviving congenital diaphragmatic hernia (CDH) suffer from anatomical and functional esophageal abnormalities. Previous work in the nitrofen animal model of CDH demonstrated malformations in neural crest-derived structures, including the vagus and recurrent laryngeal nerves. The aim of the present study was to assess whether the esophageal myenteric plexus is abnormal in rats with CDH. Methods: We used the nitrofen-induced CDH fetal rat model. Two sections of the proximal, medium and distal esophagus from both groups were processed for immunohistochemical staining with anti-neuron specific enolase and anti-S-100 antibodies; the number of stained areas was recorded for each group. Whole-mount preparations of the entire esophagus of Control and CDH animals were histochemically stained for acetylcholinesterase; the density and area of the ganglia and the number of cells/ganglia were determined. Comparisons between groups were made by standard statistical methods. Results: The number of immunohistochemically stained areas in transversal sections were decreased in CDH animals for anti-enolase (11.5±6.06 vs. 1.93±1.49, control vs. CDH, p <0.001) and anti S-100 antibodies (8.57±4.1 vs. 4.06±2.82, p <0.001). In whole-mount preparations the number of ganglia per high power field (35.16±6.57 vs. 29.29±10.26, p <0.05), the number of cells per ganglia (11.85±3.52 vs. 2.28±4.61, p <0.0001) and the relative area of the ganglia (0.35±0.32 vs. 0.18±0.42 %, p

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalEuropean Journal of Pediatric Surgery
Issue number3
Publication statusPublished - Jun 2009


  • Congenital diaphragmatic hernia
  • Enteric nervous system
  • Esophagus
  • Neural crest
  • Nitrofen

ASJC Scopus subject areas

  • Medicine(all)
  • Pediatrics, Perinatology, and Child Health
  • Surgery


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