TY - GEN
T1 - The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia
AU - Secondo, Agnese
AU - Staiano, Ilaria Rosaria
AU - Scorziello, Antonella
AU - Sirabella, Rossana
AU - Boscia, Francesca
AU - Adornetto, Annagrazia
AU - Canzoniero, Lorella Maria Teresa
AU - Di Renzo, Gianfranco
AU - Annunziato, Lucio
PY - 2007/3
Y1 - 2007/3
N2 - Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.
AB - Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.
KW - [Ca] homeostasis
KW - Chemical hypoxia
KW - Na/Ca exchanger
KW - Neuroprotection
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UR - http://www.scopus.com/inward/citedby.url?scp=34248524403&partnerID=8YFLogxK
U2 - 10.1196/annals.1387.052
DO - 10.1196/annals.1387.052
M3 - Conference contribution
C2 - 17446490
AN - SCOPUS:34248524403
SN - 1573316490
SN - 9781573316491
VL - 1099
T3 - Annals of the New York Academy of Sciences
SP - 481
EP - 485
BT - Annals of the New York Academy of Sciences
ER -