The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia

Agnese Secondo, Ilaria Rosaria Staiano, Antonella Scorziello, Rossana Sirabella, Francesca Boscia, Annagrazia Adornetto, Lorella Maria Teresa Canzoniero, Gianfranco Di Renzo, Lucio Annunziato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages481-485
Number of pages5
Volume1099
DOIs
Publication statusPublished - Mar 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1099
ISSN (Print)00778923
ISSN (Electronic)17496632

Keywords

  • [Ca] homeostasis
  • Chemical hypoxia
  • Na/Ca exchanger
  • Neuroprotection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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