The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia

Agnese Secondo; Ilaria Rosaria Staiano; Antonella Scorziello; Rossana Sirabella; Francesca Boscia; Annagrazia Adornetto; Lorella Maria Teresa Canzoniero; Gianfranco Di Renzo; Lucio Annunziato

doi:10.1196/annals.1387.052

The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia

Agnese Secondo, Ilaria Rosaria Staiano, Antonella Scorziello, Rossana Sirabella, Francesca Boscia, Annagrazia Adornetto, Lorella Maria Teresa Canzoniero, Gianfranco Di Renzo, Lucio Annunziato

IRCCS SDN

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Chemical hypoxia produces depletion of ATP, intracellular Ca²⁺ overload, and cell death. The role of Na⁺/Ca²⁺ exchanger (NCX), the major plasma membrane Ca²⁺ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca²⁺]_i increase evoked by Na⁺-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.

Original language	English
Title of host publication	Annals of the New York Academy of Sciences
Pages	481-485
Number of pages	5
Volume	1099
DOIs	https://doi.org/10.1196/annals.1387.052
Publication status	Published - Mar 2007

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1099
ISSN (Print)	00778923
ISSN (Electronic)	17496632

Keywords

[Ca] homeostasis
Chemical hypoxia
Na/Ca exchanger
Neuroprotection

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1196/annals.1387.052

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Secondo, A., Staiano, I. R., Scorziello, A., Sirabella, R., Boscia, F., Adornetto, A., Canzoniero, L. M. T., Di Renzo, G., & Annunziato, L. (2007). The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia. In Annals of the New York Academy of Sciences (Vol. 1099, pp. 481-485). (Annals of the New York Academy of Sciences; Vol. 1099). https://doi.org/10.1196/annals.1387.052

The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia. / Secondo, Agnese; Staiano, Ilaria Rosaria; Scorziello, Antonella; Sirabella, Rossana; Boscia, Francesca; Adornetto, Annagrazia; Canzoniero, Lorella Maria Teresa; Di Renzo, Gianfranco; Annunziato, Lucio.

Annals of the New York Academy of Sciences. Vol. 1099 2007. p. 481-485 (Annals of the New York Academy of Sciences; Vol. 1099).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Secondo, A, Staiano, IR, Scorziello, A, Sirabella, R, Boscia, F, Adornetto, A, Canzoniero, LMT, Di Renzo, G & Annunziato, L 2007, The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia. in Annals of the New York Academy of Sciences. vol. 1099, Annals of the New York Academy of Sciences, vol. 1099, pp. 481-485. https://doi.org/10.1196/annals.1387.052

Secondo A, Staiano IR, Scorziello A, Sirabella R, Boscia F, Adornetto A et al. The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia. In Annals of the New York Academy of Sciences. Vol. 1099. 2007. p. 481-485. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1387.052

Secondo, Agnese ; Staiano, Ilaria Rosaria ; Scorziello, Antonella ; Sirabella, Rossana ; Boscia, Francesca ; Adornetto, Annagrazia ; Canzoniero, Lorella Maria Teresa ; Di Renzo, Gianfranco ; Annunziato, Lucio. / The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia. Annals of the New York Academy of Sciences. Vol. 1099 2007. pp. 481-485 (Annals of the New York Academy of Sciences).

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abstract = "Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.",

keywords = "[Ca] homeostasis, Chemical hypoxia, Na/Ca exchanger, Neuroprotection",

author = "Agnese Secondo and Staiano, {Ilaria Rosaria} and Antonella Scorziello and Rossana Sirabella and Francesca Boscia and Annagrazia Adornetto and Canzoniero, {Lorella Maria Teresa} and {Di Renzo}, Gianfranco and Lucio Annunziato",

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AU - Secondo, Agnese

AU - Staiano, Ilaria Rosaria

AU - Scorziello, Antonella

AU - Sirabella, Rossana

AU - Boscia, Francesca

AU - Adornetto, Annagrazia

AU - Canzoniero, Lorella Maria Teresa

AU - Di Renzo, Gianfranco

AU - Annunziato, Lucio

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N2 - Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.

AB - Chemical hypoxia produces depletion of ATP, intracellular Ca2+ overload, and cell death. The role of Na+/Ca2+ exchanger (NCX), the major plasma membrane Ca2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.

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The Na+/Ca2+ exchanger isoform 3 (NCX3) but not isoform 2 (NCX2) and 1 (NCX1) singly transfected in BHK cells plays a protective role in a model of in vitro hypoxia

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