The NF-κB pathway mediates fenretinide-induced apoptosis in SH-SY5Y neuroblastoma cells

Q. D. Campbell Hewson, P. E. Lovat, M. Corazzari, J. B. Catterall, C. P F Redfern

Research output: Contribution to journalArticle

Abstract

Fenretinide induces apoptosis in SH-SY5Y neuroblastoma cells via a signaling pathway involving the production of reactive oxygen species (ROS), 12-lipoxygenase activity and the induction of the GADD153 transcription factor. NF-κ B is a key element of many cell signaling pathways and adopts a pro- or anti-apoptotic role in different cell types. Studies have suggested that NF-κ B may play a pro-apoptotic role in SH-SY5Y cells, and in other cell types NF-κ B activation may be linked to lipoxygenase activity. The aim of this study was to test the hypothesis that NF-κ B activity mediates fenretinide-induced apoptosis in SH-SY5Y neuroblastoma cells. Using a dominant-negative construct for Iκ Bα stably transfected into SH-SY5Y cells, we show that apoptosis, but not the induction of ROS, in response to fenretinide was blocked by abrogation of NF-κ B activity. In parental SH-SY5Y cells, fenretinide induced NF-κ B activity and Iκ Bα phosphorylation. These results suggest that NF-κ B activity links fenretinide-induced ROS to the induction of apoptosis in SH-SH5Y cells, and may be a target for the future development of drugs for neuroblastoma therapy.

Original languageEnglish
Pages (from-to)493-498
Number of pages6
JournalApoptosis
Volume10
Issue number3
DOIs
Publication statusPublished - May 2005

Fingerprint

Fenretinide
Neuroblastoma
Apoptosis
Reactive Oxygen Species
Arachidonate 12-Lipoxygenase
Cell signaling
Phosphorylation
Lipoxygenase
Transcription Factors
Chemical activation
Pharmaceutical Preparations

Keywords

  • Apoptosis
  • Fenretinide
  • Neuroblastoma
  • NF-κ B
  • Retinoic acid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Campbell Hewson, Q. D., Lovat, P. E., Corazzari, M., Catterall, J. B., & Redfern, C. P. F. (2005). The NF-κB pathway mediates fenretinide-induced apoptosis in SH-SY5Y neuroblastoma cells. Apoptosis, 10(3), 493-498. https://doi.org/10.1007/s10495-005-1878-z

The NF-κB pathway mediates fenretinide-induced apoptosis in SH-SY5Y neuroblastoma cells. / Campbell Hewson, Q. D.; Lovat, P. E.; Corazzari, M.; Catterall, J. B.; Redfern, C. P F.

In: Apoptosis, Vol. 10, No. 3, 05.2005, p. 493-498.

Research output: Contribution to journalArticle

Campbell Hewson, QD, Lovat, PE, Corazzari, M, Catterall, JB & Redfern, CPF 2005, 'The NF-κB pathway mediates fenretinide-induced apoptosis in SH-SY5Y neuroblastoma cells', Apoptosis, vol. 10, no. 3, pp. 493-498. https://doi.org/10.1007/s10495-005-1878-z
Campbell Hewson QD, Lovat PE, Corazzari M, Catterall JB, Redfern CPF. The NF-κB pathway mediates fenretinide-induced apoptosis in SH-SY5Y neuroblastoma cells. Apoptosis. 2005 May;10(3):493-498. https://doi.org/10.1007/s10495-005-1878-z
Campbell Hewson, Q. D. ; Lovat, P. E. ; Corazzari, M. ; Catterall, J. B. ; Redfern, C. P F. / The NF-κB pathway mediates fenretinide-induced apoptosis in SH-SY5Y neuroblastoma cells. In: Apoptosis. 2005 ; Vol. 10, No. 3. pp. 493-498.
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