The normal erbB-2 product is an atypical receptor-like tyrosine kinase with constitutive activity in the absence of ligand

F. Lonardo, E. Di Marco, C. R. King, J. H. Pierce, O. Segatto, S. A. Aaronson, P. P. Di Fiore

Research output: Contribution to journalArticle

Abstract

Overexpression of the erbB-2/neu gene is frequently detected in human cancers. When overexpressed in NIH 3T3 cells, the normal erbB-2 product, gp185(erbB-2), displays potent transforming ability as well as constitutively elevated levels of tyrosine kinase activity in the absence of exogenously added ligand. To investigate the basis for its chronic activation we sought evidence of a ligand for gp185(erbB-2) either in serum or produced by NIH 3T3 cells in an autocrine manner. We demonstrate that a putative ligand for gp185(erbB-2) is not contained in serum. Chimeric molecules composed of the extracellular domain of gp185(erbB-2) and the intracellular portion of the epidermal growth factor receptor (EGFR) did not show any transforming ability or constitutive autophosphorylation when they were expressed in NIH 3T3 cells. However, they were able to transduce a mitogenic signal when triggered by a monoclonal antibody directed against the extracellular domain of erbB-2. These results provide evidence against the idea that an erbB-2 ligand is produced by NIH 3T3 cells. Furthermore, we obtained direct evidence of the constitutive enzymative activity of gp185(erbB-2) by demonstrating that the erbB-2 kinase remained active in a chimeric configuration with the extracellular domain of the EGFR, in the absence of any detectable ligand for the EGFR. Thus, under conditions of overexpression, the normal gp185(erbB-2) is a constitutively active kinase able to transform NIH 3T3 cells in the absence of ligand.

Original languageEnglish
Pages (from-to)992-1003
Number of pages12
JournalNew Biologist
Volume2
Issue number11
Publication statusPublished - 1990

Fingerprint

Receptor Protein-Tyrosine Kinases
NIH 3T3 Cells
Protein-Tyrosine Kinases
Ligands
Epidermal Growth Factor Receptor
erbB-2 Genes
Phosphotransferases
Serum
Genes
Chemical activation
Monoclonal Antibodies
Molecules
Neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The normal erbB-2 product is an atypical receptor-like tyrosine kinase with constitutive activity in the absence of ligand. / Lonardo, F.; Di Marco, E.; King, C. R.; Pierce, J. H.; Segatto, O.; Aaronson, S. A.; Di Fiore, P. P.

In: New Biologist, Vol. 2, No. 11, 1990, p. 992-1003.

Research output: Contribution to journalArticle

@article{556c1cd8d7ea4441b3bc6352faf7c3f3,
title = "The normal erbB-2 product is an atypical receptor-like tyrosine kinase with constitutive activity in the absence of ligand",
abstract = "Overexpression of the erbB-2/neu gene is frequently detected in human cancers. When overexpressed in NIH 3T3 cells, the normal erbB-2 product, gp185(erbB-2), displays potent transforming ability as well as constitutively elevated levels of tyrosine kinase activity in the absence of exogenously added ligand. To investigate the basis for its chronic activation we sought evidence of a ligand for gp185(erbB-2) either in serum or produced by NIH 3T3 cells in an autocrine manner. We demonstrate that a putative ligand for gp185(erbB-2) is not contained in serum. Chimeric molecules composed of the extracellular domain of gp185(erbB-2) and the intracellular portion of the epidermal growth factor receptor (EGFR) did not show any transforming ability or constitutive autophosphorylation when they were expressed in NIH 3T3 cells. However, they were able to transduce a mitogenic signal when triggered by a monoclonal antibody directed against the extracellular domain of erbB-2. These results provide evidence against the idea that an erbB-2 ligand is produced by NIH 3T3 cells. Furthermore, we obtained direct evidence of the constitutive enzymative activity of gp185(erbB-2) by demonstrating that the erbB-2 kinase remained active in a chimeric configuration with the extracellular domain of the EGFR, in the absence of any detectable ligand for the EGFR. Thus, under conditions of overexpression, the normal gp185(erbB-2) is a constitutively active kinase able to transform NIH 3T3 cells in the absence of ligand.",
author = "F. Lonardo and {Di Marco}, E. and King, {C. R.} and Pierce, {J. H.} and O. Segatto and Aaronson, {S. A.} and {Di Fiore}, {P. P.}",
year = "1990",
language = "English",
volume = "2",
pages = "992--1003",
journal = "New Biologist",
issn = "1043-4674",
number = "11",

}

TY - JOUR

T1 - The normal erbB-2 product is an atypical receptor-like tyrosine kinase with constitutive activity in the absence of ligand

AU - Lonardo, F.

AU - Di Marco, E.

AU - King, C. R.

AU - Pierce, J. H.

AU - Segatto, O.

AU - Aaronson, S. A.

AU - Di Fiore, P. P.

PY - 1990

Y1 - 1990

N2 - Overexpression of the erbB-2/neu gene is frequently detected in human cancers. When overexpressed in NIH 3T3 cells, the normal erbB-2 product, gp185(erbB-2), displays potent transforming ability as well as constitutively elevated levels of tyrosine kinase activity in the absence of exogenously added ligand. To investigate the basis for its chronic activation we sought evidence of a ligand for gp185(erbB-2) either in serum or produced by NIH 3T3 cells in an autocrine manner. We demonstrate that a putative ligand for gp185(erbB-2) is not contained in serum. Chimeric molecules composed of the extracellular domain of gp185(erbB-2) and the intracellular portion of the epidermal growth factor receptor (EGFR) did not show any transforming ability or constitutive autophosphorylation when they were expressed in NIH 3T3 cells. However, they were able to transduce a mitogenic signal when triggered by a monoclonal antibody directed against the extracellular domain of erbB-2. These results provide evidence against the idea that an erbB-2 ligand is produced by NIH 3T3 cells. Furthermore, we obtained direct evidence of the constitutive enzymative activity of gp185(erbB-2) by demonstrating that the erbB-2 kinase remained active in a chimeric configuration with the extracellular domain of the EGFR, in the absence of any detectable ligand for the EGFR. Thus, under conditions of overexpression, the normal gp185(erbB-2) is a constitutively active kinase able to transform NIH 3T3 cells in the absence of ligand.

AB - Overexpression of the erbB-2/neu gene is frequently detected in human cancers. When overexpressed in NIH 3T3 cells, the normal erbB-2 product, gp185(erbB-2), displays potent transforming ability as well as constitutively elevated levels of tyrosine kinase activity in the absence of exogenously added ligand. To investigate the basis for its chronic activation we sought evidence of a ligand for gp185(erbB-2) either in serum or produced by NIH 3T3 cells in an autocrine manner. We demonstrate that a putative ligand for gp185(erbB-2) is not contained in serum. Chimeric molecules composed of the extracellular domain of gp185(erbB-2) and the intracellular portion of the epidermal growth factor receptor (EGFR) did not show any transforming ability or constitutive autophosphorylation when they were expressed in NIH 3T3 cells. However, they were able to transduce a mitogenic signal when triggered by a monoclonal antibody directed against the extracellular domain of erbB-2. These results provide evidence against the idea that an erbB-2 ligand is produced by NIH 3T3 cells. Furthermore, we obtained direct evidence of the constitutive enzymative activity of gp185(erbB-2) by demonstrating that the erbB-2 kinase remained active in a chimeric configuration with the extracellular domain of the EGFR, in the absence of any detectable ligand for the EGFR. Thus, under conditions of overexpression, the normal gp185(erbB-2) is a constitutively active kinase able to transform NIH 3T3 cells in the absence of ligand.

UR - http://www.scopus.com/inward/record.url?scp=0025687686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025687686&partnerID=8YFLogxK

M3 - Article

C2 - 1983208

AN - SCOPUS:0025687686

VL - 2

SP - 992

EP - 1003

JO - New Biologist

JF - New Biologist

SN - 1043-4674

IS - 11

ER -