The novel diterpene 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone from Salvia corrugata shows complex cytotoxic activities against human breast epithelial cells

Katia Cortese, Silvia Marconi, Carolina D'Alesio, Daniela Calzia, Isabella Panfoli, Sara Tavella, Cinzia Aiello, Francesca Pedrelli, Angela Bisio, Patrizio Castagnola

Research output: Contribution to journalArticle

Abstract

Aims: The aim of this study was the characterization of the in vitro cytotoxic properties of a recently isolated diterpene compound, 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone (compound 1), extracted from Salvia corrugata, versus human cell lines. Main methods: We used as model study immortalized breast epithelial cells MCF10A and two ERBB2+ breast cancer (BCa) cell lines, SKBR-3 and BT474. Compound 1 was isolated by methanolic extraction from regenerated shoots of Salvia corrugata Vahl, and purified by high pressure liquid chromatography (HPLC). Flow cytometry (FCM) was employed for cell cycle, apoptosis and reactive oxygen species (ROS) analysis. Cell morphology was assessed by immunofluorescence and transmission electron microscopy (TEM). Key findings: Compound 1 inhibited cell survival of all breast cell lines. In particular, compound 1 promoted cell cycle arrest in the G0/G1 phase and apoptosis along with impairment of the mitochondrial function, which was reflected in a gross alteration of the mitochondrial network structure. Furthermore, we also detected a potent activation of the ERK1/2 kinase, which suggested the induction of reactive oxygen species (ROS). Partial rescue of survival obtained with n-acetylcysteine (NAC) when coadminstered with compound 1 further supported a contribution of ROS mediated mechanisms to the growth-arrest and proapoptotic activity of compound 1 in both BCa cell lines. ROS production was indeed confirmed in SKBR-3. Significance: Our findings show that compound 1 has a cytotoxic activity against both human normal and cancer cell lines derived from breast epithelia, which is mediated by ROS generation and mitochondrial damage.

Original languageEnglish
Article number116610
JournalLife Sciences
Volume232
DOIs
Publication statusPublished - Sep 1 2019

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Salvia
Diterpenes
Human Activities
Reactive Oxygen Species
Breast
Epithelial Cells
Cells
Cell Line
Apoptosis
Breast Neoplasms
Cell Cycle Resting Phase
Mitogen-Activated Protein Kinase 3
Acetylcysteine
G1 Phase
Cell Cycle Checkpoints
Transmission Electron Microscopy
High pressure liquid chromatography
Fluorescent Antibody Technique
Cell Survival
Cell Cycle

Keywords

  • Breast
  • Diterpenes
  • ERBB2
  • ERK1/2
  • Mitochondria
  • ROS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

The novel diterpene 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone from Salvia corrugata shows complex cytotoxic activities against human breast epithelial cells. / Cortese, Katia; Marconi, Silvia; D'Alesio, Carolina; Calzia, Daniela; Panfoli, Isabella; Tavella, Sara; Aiello, Cinzia; Pedrelli, Francesca; Bisio, Angela; Castagnola, Patrizio.

In: Life Sciences, Vol. 232, 116610, 01.09.2019.

Research output: Contribution to journalArticle

Cortese, Katia ; Marconi, Silvia ; D'Alesio, Carolina ; Calzia, Daniela ; Panfoli, Isabella ; Tavella, Sara ; Aiello, Cinzia ; Pedrelli, Francesca ; Bisio, Angela ; Castagnola, Patrizio. / The novel diterpene 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone from Salvia corrugata shows complex cytotoxic activities against human breast epithelial cells. In: Life Sciences. 2019 ; Vol. 232.
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AU - Cortese, Katia

AU - Marconi, Silvia

AU - D'Alesio, Carolina

AU - Calzia, Daniela

AU - Panfoli, Isabella

AU - Tavella, Sara

AU - Aiello, Cinzia

AU - Pedrelli, Francesca

AU - Bisio, Angela

AU - Castagnola, Patrizio

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AB - Aims: The aim of this study was the characterization of the in vitro cytotoxic properties of a recently isolated diterpene compound, 7β-acetoxy-20-hydroxy-19,20-epoxyroyleanone (compound 1), extracted from Salvia corrugata, versus human cell lines. Main methods: We used as model study immortalized breast epithelial cells MCF10A and two ERBB2+ breast cancer (BCa) cell lines, SKBR-3 and BT474. Compound 1 was isolated by methanolic extraction from regenerated shoots of Salvia corrugata Vahl, and purified by high pressure liquid chromatography (HPLC). Flow cytometry (FCM) was employed for cell cycle, apoptosis and reactive oxygen species (ROS) analysis. Cell morphology was assessed by immunofluorescence and transmission electron microscopy (TEM). Key findings: Compound 1 inhibited cell survival of all breast cell lines. In particular, compound 1 promoted cell cycle arrest in the G0/G1 phase and apoptosis along with impairment of the mitochondrial function, which was reflected in a gross alteration of the mitochondrial network structure. Furthermore, we also detected a potent activation of the ERK1/2 kinase, which suggested the induction of reactive oxygen species (ROS). Partial rescue of survival obtained with n-acetylcysteine (NAC) when coadminstered with compound 1 further supported a contribution of ROS mediated mechanisms to the growth-arrest and proapoptotic activity of compound 1 in both BCa cell lines. ROS production was indeed confirmed in SKBR-3. Significance: Our findings show that compound 1 has a cytotoxic activity against both human normal and cancer cell lines derived from breast epithelia, which is mediated by ROS generation and mitochondrial damage.

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KW - Diterpenes

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KW - ERK1/2

KW - Mitochondria

KW - ROS

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