The nuclear RNA-binding protein Sam68 translocates to the cytoplasm and associates with the polysemes in mouse spermatocytes

Maria Paola Paronetto, Francesca Zalfa, Flavia Botti, Raffaele Geremia, Claudia Bagni, Claudio Sette

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Translational control plays a crucial role during gametogenesis in organisms as different as worms and mammals. Mouse knockout models have highlighted the essential function of many RNA-binding proteins during spermatogenesis. Herein we have investigated the expression and function during mammalian male meiosis of Sam68, an RNA-binding protein implicated in several aspects of RNA metabolism. Sam68 expression and localization within the cells is stage specific: it is expressed in the nucleus of spermatogonia, it disappears at the onset of meiosis (leptotene/zygotene stages), and it accumulates again in the nucleus of pachytene spermatocytes and round spermatids. During the meiotic divisions, Sam68 translocates to the cytoplasm where it is found associated with the polysomes. Translocation correlates with serine/threonine phosphorylation and it is blocked by inhibitors of the mitogen activated protein kinases ERK1/2 and of the maturation promoting factor cyclinB-cdc2 complex. Both kinases associate with Sam68 in pachytene spermatocytes and phosphorylate the regulatory regions upstream and downstream of the Sam68 RNA-binding motif. Molecular cloning of the mRNAs associated with Sam68 in mouse spermatocytes reveals a subset of genes that might be posttranscriptionally regulated by this RNA-binding protein during spermatogenesis. We also demonstrate that Sam68 shuttles between the nucleus and the cytoplasm in secondary spermatocytes, suggesting that it may promote translation of specific RNA targets during the meiotic divisions.

Original languageEnglish
Pages (from-to)14-24
Number of pages11
JournalMolecular Biology of the Cell
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 2006

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Nuclear RNA
Spermatocytes
RNA-Binding Proteins
Nuclear Proteins
Cytoplasm
Meiotic Prophase I
Meiosis
Spermatogenesis
Maturation-Promoting Factor
RNA
Gametogenesis
Spermatogonia
Polyribosomes
Spermatids
Nucleic Acid Regulatory Sequences
Mitogen-Activated Protein Kinase 1
Molecular Cloning
Threonine
Knockout Mice
Serine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The nuclear RNA-binding protein Sam68 translocates to the cytoplasm and associates with the polysemes in mouse spermatocytes. / Paronetto, Maria Paola; Zalfa, Francesca; Botti, Flavia; Geremia, Raffaele; Bagni, Claudia; Sette, Claudio.

In: Molecular Biology of the Cell, Vol. 17, No. 1, 01.2006, p. 14-24.

Research output: Contribution to journalArticle

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