The Nutraceutical Dehydrozingerone and Its Dimer Counteract Inflammation- and Oxidative Stress-Induced Dysfunction of In Vitro Cultured Human Endothelial Cells: A Novel Perspective for the Prevention and Therapy of Atherosclerosis

Elisabetta Profumo, Brigitta Buttari, Daniela D'Arcangelo, Lavinia Tinaburri, Maria Antonietta Dettori, Davide Fabbri, Giovanna Delogu, Rachele Riganò

Research output: Contribution to journalArticle

Abstract

Atherosclerosis is characterized by endothelial dysfunction, mainly induced by inflammation and oxidative stress. Increased reactive oxygen species (ROS) production together with increased adhesion molecules and thrombogenic tissue factor (TF) expression on endothelial cells has a key role in proatherogenic mechanisms. Therefore downmodulation of these molecules could be useful for reducing the severity of inflammation and atherosclerosis progression. Dehydrozingerone (DHZ) is a nutraceutical compound with anti-inflammatory and antioxidant activities. In this study we evaluated the ability of DHZ and its symmetric dimer to modulate hydrogen peroxide- (H2O2-) induced ROS production in human umbilical vein endothelial cells (HUVEC). We also evaluated intercellular adhesion molecule- (ICAM-) 1, vascular cell adhesion molecule- (VCAM-) 1, and TF expression in HUVEC activated by tumor necrosis factor- (TNF-) α. HUVEC pretreatment with DHZ and DHZ dimer reduced H2O2-induced ROS production and inhibited adhesion molecule expression and secretion. Of note, only DHZ dimer was able to reduce TF expression. DHZ effects were in part mediated by the inhibition of the nuclear factor- (NF-) κB activation. Overall, our findings demonstrate that the DHZ dimer exerts a potent anti-inflammatory, antioxidant, and antithrombotic activity on endothelial cells and suggest potential usefulness of this compound to contrast the pathogenic mechanisms involved in atherosclerosis progression.

Original languageEnglish
Pages (from-to)1246485
JournalOxidative Medicine and Cellular Longevity
Volume2016
DOIs
Publication statusPublished - 2016

Keywords

  • Atherosclerosis
  • Cell Adhesion
  • Cell Survival
  • Dietary Supplements
  • Dimerization
  • Fluorometry
  • Gene Expression Regulation
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Inflammation
  • Intercellular Adhesion Molecule-1
  • Intracellular Space
  • NF-kappa B
  • Oxidative Stress
  • Reactive Oxygen Species
  • Solubility
  • Styrenes
  • Thromboplastin
  • Vascular Cell Adhesion Molecule-1
  • Journal Article

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