Murine splenocytes contain 2 minor subpopulations of B cells, one inducible by lipopolysaccharide to convert within 2 hr from IgD- to IgD+ and the other to change from IgD+ to IgD-. These 2 subpopulations can be separated by density centrifugation. Their relative proportions show a marked age dependency: IgD- → IgD+ cells are more frequent in suckling mice, while IgD+ → IgD- inducible cells become predominant in mice older than 3 wk. The age dependency observed in the relative proportions between the 2 cell types suggests that they are ontogenetically related as progenitor-successor. This hypothesis is corroborated by phenotype analysis of the 2 subsets, revealing IgD- → IgD+ cells as IgM+, Ia+, complement receptor- (CR-) and IgD+ → IgD- cells as IgM+, Ia+, CR+. The authors' data show that IgD and CR are expressed concomitantly during B cell differentiation. On further differentiation, induced by lipopolysaccharide, both markers are lost from the cell surface at different rates: IgD decreases significantly in a very short period (8 hr). The loss of IgD may thus herald an early differentiation event toward antibody-producing cells.
|Number of pages||6|
|Journal||European Journal of Immunology|
|Publication status||Published - 1979|
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