The organic selenium compound selenomethionine modulates bleomycin-induced DNA damage and repair in human leukocytes

Blanca Laffon, Vanessa Valdiglesias, Eduardo Pásaro, Josefina Méndez

Research output: Contribution to journalArticle

Abstract

The objective of this work was to evaluate the effects of selenomethionine (SeMet) on the induction, repair, and persistence of DNA damage in human leukocytes challenged with bleomycin (BLM). Comet assay was used to determine DNA strand breaks and hOGG1 for the specific recognition of oxidative damage. Leukocytes were (A) stimulated with phytohemagglutinin, (B) damaged with BLM, and (C) incubated to allow DNA repair. Comet assay was performed after each phase. SeMet (50 μM) was supplemented either during phase A, B, or C, or AB, or ABC. Treatment with SeMet decreased BLM-induced stand breaks when added during phase AB. Results obtained after the repair period indicate that SeMet favors repair of DNA damage especially when applied during phase AB. The comparison between DNA damage before and after repair showed that BLM-induced damage was repaired better in the presence of SeMet. Our results showed antigenotoxic effect of SeMet on BLM-induced DNA and also on repair and persistence of this damage when applied before and simultaneously with BLM.

Original languageEnglish
Pages (from-to)12-19
Number of pages8
JournalBiological Trace Element Research
Volume133
Issue number1
DOIs
Publication statusPublished - Jan 2010

Keywords

  • Antigenotoxicity
  • Bleomycin
  • Comet assay
  • Oxidative damage
  • Selenomethionine

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Inorganic Chemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

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