TY - JOUR
T1 - The outer capsid glycoprotein VP7 of simian rotavirus SA11 contains two distinct neutralization epitopes
AU - Gerna, G.
AU - Sarasini, A.
AU - Di Matteo, A.
AU - Passarani, N.
AU - Gagliardi, V.
AU - Milanesi, G.
AU - Astaldi Ricotti, G. C B
AU - Battaglia, M.
PY - 1988
Y1 - 1988
N2 - Seven neutralizing monoclonal antibodies (MAbs) to the rotavirus simian agent 11 were produced. Although displaying variable degrees of haemagglutination-inhibiting activity, they were shown by radioimmunoprecipitation and Western blot analyses to react with the major outer capsid glycoprotein (VP7). In competition binding assays, MAbs defined two distinct VP7 epitopes, which appeared to be close to each other or partially overlapping. In addition, MAbs of the two epitope groups enhanced binding of a broadly reactive, non-neutralizing, MAb specific for rotavirus group antigen.
AB - Seven neutralizing monoclonal antibodies (MAbs) to the rotavirus simian agent 11 were produced. Although displaying variable degrees of haemagglutination-inhibiting activity, they were shown by radioimmunoprecipitation and Western blot analyses to react with the major outer capsid glycoprotein (VP7). In competition binding assays, MAbs defined two distinct VP7 epitopes, which appeared to be close to each other or partially overlapping. In addition, MAbs of the two epitope groups enhanced binding of a broadly reactive, non-neutralizing, MAb specific for rotavirus group antigen.
UR - http://www.scopus.com/inward/record.url?scp=0023929190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023929190&partnerID=8YFLogxK
M3 - Article
C2 - 2451709
AN - SCOPUS:0023929190
VL - 69
SP - 937
EP - 944
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 4
ER -