The outer capsid glycoprotein VP7 of simian rotavirus SA11 contains two distinct neutralization epitopes

G. Gerna, A. Sarasini, A. Di Matteo, N. Passarani, V. Gagliardi, G. Milanesi, G. C B Astaldi Ricotti, M. Battaglia

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Seven neutralizing monoclonal antibodies (MAbs) to the rotavirus simian agent 11 were produced. Although displaying variable degrees of haemagglutination-inhibiting activity, they were shown by radioimmunoprecipitation and Western blot analyses to react with the major outer capsid glycoprotein (VP7). In competition binding assays, MAbs defined two distinct VP7 epitopes, which appeared to be close to each other or partially overlapping. In addition, MAbs of the two epitope groups enhanced binding of a broadly reactive, non-neutralizing, MAb specific for rotavirus group antigen.

Original languageEnglish
Pages (from-to)937-944
Number of pages8
JournalJournal of General Virology
Volume69
Issue number4
Publication statusPublished - 1988

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Capsid
Epitopes
Rotavirus
Monoclonal Antibodies
Hemagglutination
Neutralizing Antibodies
Glycoproteins
Western Blotting
Antigens
Rotavirus VP7 protein

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The outer capsid glycoprotein VP7 of simian rotavirus SA11 contains two distinct neutralization epitopes. / Gerna, G.; Sarasini, A.; Di Matteo, A.; Passarani, N.; Gagliardi, V.; Milanesi, G.; Astaldi Ricotti, G. C B; Battaglia, M.

In: Journal of General Virology, Vol. 69, No. 4, 1988, p. 937-944.

Research output: Contribution to journalArticle

Gerna, G, Sarasini, A, Di Matteo, A, Passarani, N, Gagliardi, V, Milanesi, G, Astaldi Ricotti, GCB & Battaglia, M 1988, 'The outer capsid glycoprotein VP7 of simian rotavirus SA11 contains two distinct neutralization epitopes', Journal of General Virology, vol. 69, no. 4, pp. 937-944.
Gerna, G. ; Sarasini, A. ; Di Matteo, A. ; Passarani, N. ; Gagliardi, V. ; Milanesi, G. ; Astaldi Ricotti, G. C B ; Battaglia, M. / The outer capsid glycoprotein VP7 of simian rotavirus SA11 contains two distinct neutralization epitopes. In: Journal of General Virology. 1988 ; Vol. 69, No. 4. pp. 937-944.
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