The oxido-redox potential of albumin. Methodological approach and relevance to human diseases

Giovanni Candiano, Andrea Petretto, Maurizio Bruschi, Laura Santucci, Veronica Dimuccio, Marco Prunotto, Rosanna Gusmano, Andrea Urbani, Gian Marco Ghiggeri

Research output: Contribution to journalArticle

Abstract

In humans, an increased synthesis of reactive oxygen species (ROS) may be a relevant cause of amplification of physiologic processes resulting in inflammatory organ damage or neoplasia. Efficient anti-oxidative systems targeting oxidative stress are thus essential to prevent tissue damage. In plasma, proteins proved to be the first line of defence against ROS and albumin, the highest concentration plasma protein, has a key role in this antioxidant function. Recent studies have clearly documented that albumin oxido-redox potential changes upon oxidation by different oxidants thus becoming a deputy biomarker of this process. ROS react primarily with the free 34Cysteine (34Cys) residue of albumin to form two reversible intermediate derivatives, sulfenic-(SOH-alb) and sulfinic acid (SO2H-alb), resulting in sulfonic acid (SO3H-alb), the final stable product of the reaction. Upon stable oxidation (SO3H-alb), albumin properties are altered: the protein becomes more susceptible to trypsin digestion and is degraded faster compared to the non-oxidized counterpart. The present review focuses on the characterization of albumin chemical changes induced by ROS, their relevance in human pathology and the most recent advances in the approach to oxidation adduct analysis.

Original languageEnglish
Pages (from-to)188-195
Number of pages8
JournalJournal of Proteomics
Volume73
Issue number2
DOIs
Publication statusPublished - Dec 1 2009

Fingerprint

Oxidation-Reduction
Albumins
Reactive Oxygen Species
Oxidation
Blood Proteins
Sulfinic Acids
Oxidative stress
Sulfonic Acids
Biomarkers
Pathology
Oxidants
Trypsin
Amplification
Digestion
Proteins
Oxidative Stress
Antioxidants
Tissue
Derivatives
Neoplasms

Keywords

  • 2D native SDS-PAGE
  • 2D-DIGE
  • Focal segmental glomerulosclerosis
  • Oxido-redox potential
  • Plasma albumin
  • Protein oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

The oxido-redox potential of albumin. Methodological approach and relevance to human diseases. / Candiano, Giovanni; Petretto, Andrea; Bruschi, Maurizio; Santucci, Laura; Dimuccio, Veronica; Prunotto, Marco; Gusmano, Rosanna; Urbani, Andrea; Ghiggeri, Gian Marco.

In: Journal of Proteomics, Vol. 73, No. 2, 01.12.2009, p. 188-195.

Research output: Contribution to journalArticle

Candiano, Giovanni ; Petretto, Andrea ; Bruschi, Maurizio ; Santucci, Laura ; Dimuccio, Veronica ; Prunotto, Marco ; Gusmano, Rosanna ; Urbani, Andrea ; Ghiggeri, Gian Marco. / The oxido-redox potential of albumin. Methodological approach and relevance to human diseases. In: Journal of Proteomics. 2009 ; Vol. 73, No. 2. pp. 188-195.
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