Abstract
In mouse mammary epithelial C127 cells expressing wild-type cystic fibrosis transmembrane conductance regulator (CFTR), chloride efflux, measured with the Cl- sensitive dye 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ), was stimulated by activation of protein kinase A with cyclic AMP elevating agents forskolin plus 3-isobutyl-1-methyl-xanthine (IBMX) and, to a less extent, by activation of protein kinase C with the phorbol 12-myristate 13-acetate (PMA). Conversely, bicarbonate influx, determined by intracellular alkalinization of cells incubated with the pH-sensitive dye 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluoresceintetraacetoxym ethyl ester (BCECF-AM), was stimulated by cyclic AMP elevation, but not by PMA. Patch clamp analysis revealed that PMA activated a Cl- current with the typical biophysical characteristics of swelling-activated current and not of CFTR.
Original language | English |
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Pages (from-to) | 120-127 |
Number of pages | 8 |
Journal | Biochimica et Biophysica Acta - Molecular Basis of Disease |
Volume | 1535 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 14 2001 |
Keywords
- CFTR
- Cystic fibrosis
- HCO/ influx
- Patch clamp
- Phorbol ester (PMA)
- Swelling-activated chloride current
ASJC Scopus subject areas
- Molecular Biology
- Molecular Medicine
- Biophysics