The physical map of the human RET proto-oncogene

B. Pasini, R. M W Hofstra, L. Yin, R. Bocciardi, G. Santamaria, P. M. Grootscholten, I. Ceccherini, G. Patrone, M. Priolo, C. H C M Buys, G. Romeo

Research output: Contribution to journalArticlepeer-review

Abstract

The RET proto-oncogene, a transmembrane tyrosine kinase receptor, is involved in the development of at least five different disease phenotypes. RET is activated through somatic rearrangements in a number of cases of papillary thyroid carcinoma while germ-line point mutations are associated with three inherited cancer syndromes MEN 2A, MEN 2B and FMTC. Moreover, point mutations or heterozygous deletions of BET are found in the dominant form of Hirschsprung disease or congenital colonic aganglionosis. We cloned the entire BET genomic sequence in a contig of cosmids encompassing 150 kb, from the CA repeat sTCL-2 to the region upstream the RET promoter, and established the position of the 20 exons of the RET gene with respect to a detailed restriction map based on eight endonucleases. A new highly polymorphic CA repeat sequence was identified within intron 5 of RET (RET-INT5). Finally the orientation of RET on chromosome 10q11.2 made it possible to orientate three other genes rearranged with RET in papillary thyroid carcinomas, namely H4/ D10S170 on 10q21, R1α on 17q23 and RFG2/Ele1 on 10q11.2.

Original languageEnglish
Pages (from-to)1737-1743
Number of pages7
JournalOncogene
Volume11
Issue number9
Publication statusPublished - 1995

Keywords

  • Chromosome 10
  • Gene structure
  • Hirschsprung disease
  • MEN 2
  • RET proto-oncogene

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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