TY - JOUR
T1 - The PISSLRE gene
T2 - Structure, exon skipping, and exclusion as tumor suppressor in breast cancer
AU - Crawford, Joanna
AU - Ianzano, Leonarda
AU - Savino, Maria
AU - Whitmore, Scott
AU - Cleton-Jansen, Anne Marie
AU - Settasatian, Chatri
AU - D'Apolito, Maria
AU - Seshadri, Ram
AU - Pronk, Jan C.
AU - Auerbach, Arleen D.
AU - Verlander, Peter C.
AU - Mathew, Christopher G.
AU - Tipping, Alex J.
AU - Doggett, Norman A.
AU - Zelante, Leopoldo
AU - Callen, David F.
AU - Savoia, Anna
PY - 1999/2/15
Y1 - 1999/2/15
N2 - In sporadic breast cancer, loss of heterozygosity (LOH) frequently occurs in three discrete regions of the long arm of chromosome 16q, the most telomeric of which is located at 16q24.3. Among the genes mapped to this region, PISSLRE is a plausible candidate tumor suppressor gene. It codes for a putative cyclin-dependent kinase that, as with other members of this family, is likely to be involved in regulating the cell cycle and therefore may have a role in oncogenesis. We characterized the genomic structure of PISSLRE and found that the splicing of this gene is complex. A variety of different transcripts were identified, including those due to cryptic splice sites, exon skipping, insertion of intronic sequences, and exon scrambling. The last phenomenon was observed in a rare PISSLRE transcript in which exons are joined at a nonconsensus splice site in an order different from that predicted by the genomic sequence. To screen the PISSLRE gene in breast tumors with ascertained LOH at 16q24.3, we have analyzed each exon by single- strand conformational polymorphism. No variation was found in the coding sequence, leading us to conclude that another tumor suppressor must be targeted by LOH in sporadic breast cancer.
AB - In sporadic breast cancer, loss of heterozygosity (LOH) frequently occurs in three discrete regions of the long arm of chromosome 16q, the most telomeric of which is located at 16q24.3. Among the genes mapped to this region, PISSLRE is a plausible candidate tumor suppressor gene. It codes for a putative cyclin-dependent kinase that, as with other members of this family, is likely to be involved in regulating the cell cycle and therefore may have a role in oncogenesis. We characterized the genomic structure of PISSLRE and found that the splicing of this gene is complex. A variety of different transcripts were identified, including those due to cryptic splice sites, exon skipping, insertion of intronic sequences, and exon scrambling. The last phenomenon was observed in a rare PISSLRE transcript in which exons are joined at a nonconsensus splice site in an order different from that predicted by the genomic sequence. To screen the PISSLRE gene in breast tumors with ascertained LOH at 16q24.3, we have analyzed each exon by single- strand conformational polymorphism. No variation was found in the coding sequence, leading us to conclude that another tumor suppressor must be targeted by LOH in sporadic breast cancer.
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U2 - 10.1006/geno.1998.5676
DO - 10.1006/geno.1998.5676
M3 - Article
C2 - 10036189
AN - SCOPUS:0033557438
VL - 56
SP - 90
EP - 97
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -