TY - JOUR
T1 - The plant hormone abscisic acid increases in human plasma after hyperglycemia and stimulates glucose consumption by adipocytes and myoblasts
AU - Bruzzone, Santina
AU - Ameri, Pietro
AU - Briatore, Lucia
AU - Mannino, Elena
AU - Basile, Giovanna
AU - Andraghetti, Gabriella
AU - Grozio, Alessia
AU - Magnone, Mirko
AU - Guida, Lucrezia
AU - Scarfì, Sonia
AU - Salis, Annalisa
AU - Damonte, Gianluca
AU - Sturla, Laura
AU - Nencioni, Alessio
AU - Fenoglio, Daniela
AU - Fiory, Francesca
AU - Miele, Claudia
AU - Beguinot, Francesco
AU - Ruvolo, Vittorio
AU - Bormioli, Mariano
AU - Colombo, Giuseppe
AU - Maggi, Davide
AU - Murialdo, Giovanni
AU - Cordera, Renzo
AU - De Flora, Antonio
AU - Zocchi, Elena
PY - 2012/3
Y1 - 2012/3
N2 - The plant hormone abscisic acid (ABA) is released from glucose-challenged human pancreatic β cells and stimulates insulin secretion. We investigated whether plasma ABA increased during oral and intravenous glucose tolerance tests (OGTTs and IVGTTs) in healthy human subjects. In all subjects undergoing OGTTs (n=8), plasma ABA increased over basal values (in a range from 2- to 9-fold). A positive correlation was found between the ABA area under the curve (AUC) and the glucose AUC. In 4 out of 6 IVGTTs, little or no increase of ABA levels was observed. In the remaining subjects, the ABA increase was similar to that recorded during OGTTs. GLP-1 stimulated ABA release from an insulinoma cell line and from human islets, by ∼10- and 2-fold in low and high glucose, respectively. Human adipose tissue also released ABA in response to high glucose. Nanomolar ABA stimulated glucose uptake, similarly to insulin, in rat L6 myoblasts and in murine 3T3-L1 cells differentiated to adipocytes, by increasing GLUT-4 translocation to the plasma membrane. Demonstration that a glucose load in humans is followed by a physiological rise of plasma ABA, which can enhance glucose uptake by adipose tissues and muscle cells, identifies ABA as a new mammalian hormone involved in glucose metabolism.
AB - The plant hormone abscisic acid (ABA) is released from glucose-challenged human pancreatic β cells and stimulates insulin secretion. We investigated whether plasma ABA increased during oral and intravenous glucose tolerance tests (OGTTs and IVGTTs) in healthy human subjects. In all subjects undergoing OGTTs (n=8), plasma ABA increased over basal values (in a range from 2- to 9-fold). A positive correlation was found between the ABA area under the curve (AUC) and the glucose AUC. In 4 out of 6 IVGTTs, little or no increase of ABA levels was observed. In the remaining subjects, the ABA increase was similar to that recorded during OGTTs. GLP-1 stimulated ABA release from an insulinoma cell line and from human islets, by ∼10- and 2-fold in low and high glucose, respectively. Human adipose tissue also released ABA in response to high glucose. Nanomolar ABA stimulated glucose uptake, similarly to insulin, in rat L6 myoblasts and in murine 3T3-L1 cells differentiated to adipocytes, by increasing GLUT-4 translocation to the plasma membrane. Demonstration that a glucose load in humans is followed by a physiological rise of plasma ABA, which can enhance glucose uptake by adipose tissues and muscle cells, identifies ABA as a new mammalian hormone involved in glucose metabolism.
KW - GLP-1
KW - Glucose uptake
KW - GLUT-4
KW - Insulin
KW - Oral glucose tolerance test
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UR - http://www.scopus.com/inward/citedby.url?scp=84857707217&partnerID=8YFLogxK
U2 - 10.1096/fj.11-190140
DO - 10.1096/fj.11-190140
M3 - Article
C2 - 22075645
AN - SCOPUS:84857707217
VL - 26
SP - 1251
EP - 1260
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 3
ER -