The plant hormone abscisic acid increases in human plasma after hyperglycemia and stimulates glucose consumption by adipocytes and myoblasts

Santina Bruzzone, Pietro Ameri, Lucia Briatore, Elena Mannino, Giovanna Basile, Gabriella Andraghetti, Alessia Grozio, Mirko Magnone, Lucrezia Guida, Sonia Scarfì, Annalisa Salis, Gianluca Damonte, Laura Sturla, Alessio Nencioni, Daniela Fenoglio, Francesca Fiory, Claudia Miele, Francesco Beguinot, Vittorio Ruvolo, Mariano BormioliGiuseppe Colombo, Davide Maggi, Giovanni Murialdo, Renzo Cordera, Antonio De Flora, Elena Zocchi

Research output: Contribution to journalArticlepeer-review

Abstract

The plant hormone abscisic acid (ABA) is released from glucose-challenged human pancreatic β cells and stimulates insulin secretion. We investigated whether plasma ABA increased during oral and intravenous glucose tolerance tests (OGTTs and IVGTTs) in healthy human subjects. In all subjects undergoing OGTTs (n=8), plasma ABA increased over basal values (in a range from 2- to 9-fold). A positive correlation was found between the ABA area under the curve (AUC) and the glucose AUC. In 4 out of 6 IVGTTs, little or no increase of ABA levels was observed. In the remaining subjects, the ABA increase was similar to that recorded during OGTTs. GLP-1 stimulated ABA release from an insulinoma cell line and from human islets, by ∼10- and 2-fold in low and high glucose, respectively. Human adipose tissue also released ABA in response to high glucose. Nanomolar ABA stimulated glucose uptake, similarly to insulin, in rat L6 myoblasts and in murine 3T3-L1 cells differentiated to adipocytes, by increasing GLUT-4 translocation to the plasma membrane. Demonstration that a glucose load in humans is followed by a physiological rise of plasma ABA, which can enhance glucose uptake by adipose tissues and muscle cells, identifies ABA as a new mammalian hormone involved in glucose metabolism.

Original languageEnglish
Pages (from-to)1251-1260
Number of pages10
JournalFASEB Journal
Volume26
Issue number3
DOIs
Publication statusPublished - Mar 2012

Keywords

  • GLP-1
  • Glucose uptake
  • GLUT-4
  • Insulin
  • Oral glucose tolerance test

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

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