The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose

Sonia Scarfì, Chiara Fresia, Chiara Ferraris, Santina Bruzzone, Floriana Fruscione, Cesare Usai, Federica Benvenuto, Mirko Magnone, Marina Podestà, Laura Sturla, Lucrezia Guida, Ennio Albanesi, Gianluca Damonte, Annalisa Salis, Antonio De Flora, Elena Zocchi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Abscisic acid (ABA) is a hormone involved in pivotal physiological functions in higher plants, such as response to abiotic stress and control of seed dormancy and germination. Recently, ABA was demonstrated to be autocrinally produced by human granulocytes, β pancreatic cells, and mesenchymal stem cells (MSC) and to stimulate cell-specific functions through a signaling pathway involving the second messenger cyclic ADP-ribose (cADPR). Here we show that ABA expands human uncommitted hemopoietic progenitors (HP) in vitro, through a cADPR-mediated increase of the intracellular calcium concentration ([Ca 2+]i). Incubation of CD34+ cells with micromolar ABA also induces transcriptional effects, which include NF-κB nuclear translocation and transcription of genes encoding for several cytokines. Human MSC stimulated with a lymphocyte-conditioned medium produce and release ABA at concentrations sufficient to exert growth-stimulatory effects on co-cultured CD34+ cells, as demonstrated by the inhibition of colony growth in the presence of an anti-ABA monoclonal antibody. These results provide a remarkable example of conservation of a stress hormone and of its second messenger from plants to humans and identify ABA as a new hemopoietic growth factor involved in the cross-talk between HP and MSC.

Original languageEnglish
Pages (from-to)2469-2477
Number of pages9
JournalStem Cells
Volume27
Issue number10
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Cyclic ADP-Ribose
Abscisic Acid
Plant Growth Regulators
Second Messenger Systems
Mesenchymal Stromal Cells
Plant Dormancy
Hormones
Conditioned Culture Medium
Growth
Germination
Granulocytes
Cultured Cells
Intercellular Signaling Peptides and Proteins
Monoclonal Antibodies
Lymphocytes
Cytokines
Calcium

Keywords

  • Abscisic acid
  • Cyclic ADP-ribose
  • Hemopoietic progenitors
  • Intracellular calcium
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Scarfì, S., Fresia, C., Ferraris, C., Bruzzone, S., Fruscione, F., Usai, C., ... Zocchi, E. (2009). The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose. Stem Cells, 27(10), 2469-2477. https://doi.org/10.1002/stem.173

The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose. / Scarfì, Sonia; Fresia, Chiara; Ferraris, Chiara; Bruzzone, Santina; Fruscione, Floriana; Usai, Cesare; Benvenuto, Federica; Magnone, Mirko; Podestà, Marina; Sturla, Laura; Guida, Lucrezia; Albanesi, Ennio; Damonte, Gianluca; Salis, Annalisa; De Flora, Antonio; Zocchi, Elena.

In: Stem Cells, Vol. 27, No. 10, 10.2009, p. 2469-2477.

Research output: Contribution to journalArticle

Scarfì, S, Fresia, C, Ferraris, C, Bruzzone, S, Fruscione, F, Usai, C, Benvenuto, F, Magnone, M, Podestà, M, Sturla, L, Guida, L, Albanesi, E, Damonte, G, Salis, A, De Flora, A & Zocchi, E 2009, 'The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose', Stem Cells, vol. 27, no. 10, pp. 2469-2477. https://doi.org/10.1002/stem.173
Scarfì, Sonia ; Fresia, Chiara ; Ferraris, Chiara ; Bruzzone, Santina ; Fruscione, Floriana ; Usai, Cesare ; Benvenuto, Federica ; Magnone, Mirko ; Podestà, Marina ; Sturla, Laura ; Guida, Lucrezia ; Albanesi, Ennio ; Damonte, Gianluca ; Salis, Annalisa ; De Flora, Antonio ; Zocchi, Elena. / The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose. In: Stem Cells. 2009 ; Vol. 27, No. 10. pp. 2469-2477.
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