The plasma levels of soluble HLA-G molecules correlate directly with CD34+ cell concentration and HLA-G 14bp insertion/insertion polymorphism in cord blood donors

Cristina Capittini, Paola Bergamaschi, Sara Sachetto, Mariarosa Truglio, Monica Viola, Andrea Marchesi, Valeria Genovese, Bina Romano, Marco Guarene, Rossella Poma, Miryam Martinetti, Carmine Tinelli, Laura Salvaneschi

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Cord blood provides haematopoietic stem cells for allogeneic transplantation and, thanks to the naivety of its immune system, has several advantages over other sources of stem cells. In the transplantation setting, the presence of immunosuppressive human leucocyte antigen (HLA)-G molecules has been advocated to prevent both rejection and Graft-versus-Host disease. HLA-G is physiologically expressed throughout pregnancy and is contained in cord blood at birth. Moreover, it has recently been reported that not only cord blood mesenchymal cells, but also CD34+ cell progenies produce soluble HLA-G (sHLA-G). We tried to identify the largest producer of sHLA-G among 85 healthy cord blood donors at Pavia Cord Blood Bank, correlating the sHLA-G concentration with the HLA-G 14bp insertion/deletion (INS/DEL) genotype and CD34+ cell concentration. Materials and methods. We measured sHLA-G levels in 36 cord blood plasma stored at 20°C for

Original languageEnglish
JournalBlood Transfusion
Volume12
Issue numberSUPPL.1
DOIs
Publication statusPublished - 2014

Keywords

  • CD34+ cells
  • Cord blood plasma
  • HLA-G 14bp polymorphism
  • Liquid nitrogen cryostorage
  • Soluble HLA-G

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

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