Abstract
Background. Cord blood provides haematopoietic stem cells for allogeneic transplantation and, thanks to the naivety of its immune system, has several advantages over other sources of stem cells. In the transplantation setting, the presence of immunosuppressive human leucocyte antigen (HLA)-G molecules has been advocated to prevent both rejection and Graft-versus-Host disease. HLA-G is physiologically expressed throughout pregnancy and is contained in cord blood at birth. Moreover, it has recently been reported that not only cord blood mesenchymal cells, but also CD34+ cell progenies produce soluble HLA-G (sHLA-G). We tried to identify the largest producer of sHLA-G among 85 healthy cord blood donors at Pavia Cord Blood Bank, correlating the sHLA-G concentration with the HLA-G 14bp insertion/deletion (INS/DEL) genotype and CD34+ cell concentration. Materials and methods. We measured sHLA-G levels in 36 cord blood plasma stored at 20°C for
Original language | English |
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Journal | Blood Transfusion |
Volume | 12 |
Issue number | SUPPL.1 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- CD34+ cells
- Cord blood plasma
- HLA-G 14bp polymorphism
- Liquid nitrogen cryostorage
- Soluble HLA-G
ASJC Scopus subject areas
- Hematology
- Immunology and Allergy