The plasma levels of soluble HLA-G molecules correlate directly with CD34+ cell concentration and HLA-G 14bp insertion/insertion polymorphism in cord blood donors

Cristina Capittini; Paola Bergamaschi; Sara Sachetto; Mariarosa Truglio; Monica Viola; Andrea Marchesi; Valeria Genovese; Bina Romano; Marco Guarene; Rossella Poma; Miryam Martinetti; Carmine Tinelli; Laura Salvaneschi

doi:10.2450/2012.0144-12

The plasma levels of soluble HLA-G molecules correlate directly with CD34+ cell concentration and HLA-G 14bp insertion/insertion polymorphism in cord blood donors

Cristina Capittini, Paola Bergamaschi, Sara Sachetto, Mariarosa Truglio, Monica Viola, Andrea Marchesi, Valeria Genovese, Bina Romano, Marco Guarene, Rossella Poma, Miryam Martinetti, Carmine Tinelli, Laura Salvaneschi

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Background. Cord blood provides haematopoietic stem cells for allogeneic transplantation and, thanks to the naivety of its immune system, has several advantages over other sources of stem cells. In the transplantation setting, the presence of immunosuppressive human leucocyte antigen (HLA)-G molecules has been advocated to prevent both rejection and Graft-versus-Host disease. HLA-G is physiologically expressed throughout pregnancy and is contained in cord blood at birth. Moreover, it has recently been reported that not only cord blood mesenchymal cells, but also CD34+ cell progenies produce soluble HLA-G (sHLA-G). We tried to identify the largest producer of sHLA-G among 85 healthy cord blood donors at Pavia Cord Blood Bank, correlating the sHLA-G concentration with the HLA-G 14bp insertion/deletion (INS/DEL) genotype and CD34+ cell concentration. Materials and methods. We measured sHLA-G levels in 36 cord blood plasma stored at 20°C for

Original language	English
Journal	Blood Transfusion
Volume	12
Issue number	SUPPL.1
DOIs	https://doi.org/10.2450/2012.0144-12
Publication status	Published - 2014

Keywords

CD34+ cells
Cord blood plasma
HLA-G 14bp polymorphism
Liquid nitrogen cryostorage
Soluble HLA-G

ASJC Scopus subject areas

Hematology
Immunology and Allergy

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Capittini, C., Bergamaschi, P., Sachetto, S., Truglio, M., Viola, M., Marchesi, A., Genovese, V., Romano, B., Guarene, M., Poma, R., Martinetti, M., Tinelli, C., & Salvaneschi, L. (2014). The plasma levels of soluble HLA-G molecules correlate directly with CD34+ cell concentration and HLA-G 14bp insertion/insertion polymorphism in cord blood donors. Blood Transfusion, 12(SUPPL.1). https://doi.org/10.2450/2012.0144-12

Capittini, C , Bergamaschi, P, Sachetto, S, Truglio, M, Viola, M, Marchesi, A, Genovese, V, Romano, B, Guarene, M, Poma, R, Martinetti, M, Tinelli, C & Salvaneschi, L 2014, 'The plasma levels of soluble HLA-G molecules correlate directly with CD34+ cell concentration and HLA-G 14bp insertion/insertion polymorphism in cord blood donors', Blood Transfusion, vol. 12, no. SUPPL.1. https://doi.org/10.2450/2012.0144-12

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abstract = "Background. Cord blood provides haematopoietic stem cells for allogeneic transplantation and, thanks to the naivety of its immune system, has several advantages over other sources of stem cells. In the transplantation setting, the presence of immunosuppressive human leucocyte antigen (HLA)-G molecules has been advocated to prevent both rejection and Graft-versus-Host disease. HLA-G is physiologically expressed throughout pregnancy and is contained in cord blood at birth. Moreover, it has recently been reported that not only cord blood mesenchymal cells, but also CD34+ cell progenies produce soluble HLA-G (sHLA-G). We tried to identify the largest producer of sHLA-G among 85 healthy cord blood donors at Pavia Cord Blood Bank, correlating the sHLA-G concentration with the HLA-G 14bp insertion/deletion (INS/DEL) genotype and CD34+ cell concentration. Materials and methods. We measured sHLA-G levels in 36 cord blood plasma stored at 20°C for",

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AU - Capittini, Cristina

AU - Bergamaschi, Paola

AU - Sachetto, Sara

AU - Truglio, Mariarosa

AU - Viola, Monica

AU - Marchesi, Andrea

AU - Genovese, Valeria

AU - Romano, Bina

AU - Guarene, Marco

AU - Poma, Rossella

AU - Martinetti, Miryam

AU - Tinelli, Carmine

AU - Salvaneschi, Laura

PY - 2014

Y1 - 2014

N2 - Background. Cord blood provides haematopoietic stem cells for allogeneic transplantation and, thanks to the naivety of its immune system, has several advantages over other sources of stem cells. In the transplantation setting, the presence of immunosuppressive human leucocyte antigen (HLA)-G molecules has been advocated to prevent both rejection and Graft-versus-Host disease. HLA-G is physiologically expressed throughout pregnancy and is contained in cord blood at birth. Moreover, it has recently been reported that not only cord blood mesenchymal cells, but also CD34+ cell progenies produce soluble HLA-G (sHLA-G). We tried to identify the largest producer of sHLA-G among 85 healthy cord blood donors at Pavia Cord Blood Bank, correlating the sHLA-G concentration with the HLA-G 14bp insertion/deletion (INS/DEL) genotype and CD34+ cell concentration. Materials and methods. We measured sHLA-G levels in 36 cord blood plasma stored at 20°C for

AB - Background. Cord blood provides haematopoietic stem cells for allogeneic transplantation and, thanks to the naivety of its immune system, has several advantages over other sources of stem cells. In the transplantation setting, the presence of immunosuppressive human leucocyte antigen (HLA)-G molecules has been advocated to prevent both rejection and Graft-versus-Host disease. HLA-G is physiologically expressed throughout pregnancy and is contained in cord blood at birth. Moreover, it has recently been reported that not only cord blood mesenchymal cells, but also CD34+ cell progenies produce soluble HLA-G (sHLA-G). We tried to identify the largest producer of sHLA-G among 85 healthy cord blood donors at Pavia Cord Blood Bank, correlating the sHLA-G concentration with the HLA-G 14bp insertion/deletion (INS/DEL) genotype and CD34+ cell concentration. Materials and methods. We measured sHLA-G levels in 36 cord blood plasma stored at 20°C for

KW - CD34+ cells

KW - Cord blood plasma

KW - HLA-G 14bp polymorphism

KW - Liquid nitrogen cryostorage

KW - Soluble HLA-G

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The plasma levels of soluble HLA-G molecules correlate directly with CD34+ cell concentration and HLA-G 14bp insertion/insertion polymorphism in cord blood donors

Abstract

Keywords

ASJC Scopus subject areas

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