The Plasma Membrane-associated Sialidase MmNEU3 Modifies the Ganglioside Pattern of Adjacent Cells Supporting Its Involvement in Cell-to-Cell Interactions

Nadia Papini, Luigi Anastasia, Cristina Tringali, Gianluigi Croci, Roberto Bresciani, Kazunori Yamaguchi, Taeko Miyagi, Augusto Preti, Alessandro Prinetti, Simona Prioni, Sandro Sonnino, Guido Tettamanti, Bruno Venerando, Eugenio Monti

Research output: Contribution to journalArticle

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Abstract

We describe herein the enzyme behavior of MmNEU3, the plasma membrane-associated sialidase from mouse (Mus musculus). MmNEU3 is localized at the plasma membrane as demonstrated directly by confocal microscopy analysis. In addition, administration of the radiolabeled ganglioside GD1a to MmNEU3-transfected cells, under conditions that prevent lysosomal activity, led to its hydrolysis into ganglioside GM1, further indicating the plasma membrane topology of MmNEU3. Metabolic labeling with [1-3H]sphingosine allowed the characterization of the ganglioside patterns of COS-7 cells. MmNEU3 expression in COS-7 cells led to an extensive modification of the cell ganglioside pattern, i.e. GM3 and GD1a content was decreased to about one-third compared with mock-transfected cells. At the same time, a 35% increase in ganglioside GM1 content was observed. Mixed culture of MmNEU3-transfected cells with [1-3H]sphingosine-labeled cells demonstrates that the enzyme present at the cell surface is able to recognize gangliosides exposed on the membrane of nearby cells. Under these experimental conditions, the extent of ganglioside pattern changes was a function of MmNEU3 transient expression. Overall, the variations in GM3, GD1a, and GM1 content were very similar to those observed in the case of [1-3H]sphingosine-labeled MmNEU3-transfected cells, indicating that the enzyme mainly exerted its activity toward ganglioside substrates present at the surface of neighboring cells. These results indicate that the plasma membrane-associated sialidase MmNEU3 is able to hydrolyze ganglioside substrates in intact living cells at a neutral pH, mainly through cell-to-cell interactions.

Original languageEnglish
Pages (from-to)16989-16995
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number17
DOIs
Publication statusPublished - Apr 23 2004

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Gangliosides
Neuraminidase
Cell membranes
Cell Communication
Cell Membrane
Sphingosine
G(M1) Ganglioside
Enzymes
COS Cells
Confocal microscopy
Substrates
Labeling
Hydrolysis
Cells
Topology
Membranes
Confocal Microscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Plasma Membrane-associated Sialidase MmNEU3 Modifies the Ganglioside Pattern of Adjacent Cells Supporting Its Involvement in Cell-to-Cell Interactions. / Papini, Nadia; Anastasia, Luigi; Tringali, Cristina; Croci, Gianluigi; Bresciani, Roberto; Yamaguchi, Kazunori; Miyagi, Taeko; Preti, Augusto; Prinetti, Alessandro; Prioni, Simona; Sonnino, Sandro; Tettamanti, Guido; Venerando, Bruno; Monti, Eugenio.

In: Journal of Biological Chemistry, Vol. 279, No. 17, 23.04.2004, p. 16989-16995.

Research output: Contribution to journalArticle

Papini, N, Anastasia, L, Tringali, C, Croci, G, Bresciani, R, Yamaguchi, K, Miyagi, T, Preti, A, Prinetti, A, Prioni, S, Sonnino, S, Tettamanti, G, Venerando, B & Monti, E 2004, 'The Plasma Membrane-associated Sialidase MmNEU3 Modifies the Ganglioside Pattern of Adjacent Cells Supporting Its Involvement in Cell-to-Cell Interactions', Journal of Biological Chemistry, vol. 279, no. 17, pp. 16989-16995. https://doi.org/10.1074/jbc.M400881200
Papini, Nadia ; Anastasia, Luigi ; Tringali, Cristina ; Croci, Gianluigi ; Bresciani, Roberto ; Yamaguchi, Kazunori ; Miyagi, Taeko ; Preti, Augusto ; Prinetti, Alessandro ; Prioni, Simona ; Sonnino, Sandro ; Tettamanti, Guido ; Venerando, Bruno ; Monti, Eugenio. / The Plasma Membrane-associated Sialidase MmNEU3 Modifies the Ganglioside Pattern of Adjacent Cells Supporting Its Involvement in Cell-to-Cell Interactions. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 17. pp. 16989-16995.
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abstract = "We describe herein the enzyme behavior of MmNEU3, the plasma membrane-associated sialidase from mouse (Mus musculus). MmNEU3 is localized at the plasma membrane as demonstrated directly by confocal microscopy analysis. In addition, administration of the radiolabeled ganglioside GD1a to MmNEU3-transfected cells, under conditions that prevent lysosomal activity, led to its hydrolysis into ganglioside GM1, further indicating the plasma membrane topology of MmNEU3. Metabolic labeling with [1-3H]sphingosine allowed the characterization of the ganglioside patterns of COS-7 cells. MmNEU3 expression in COS-7 cells led to an extensive modification of the cell ganglioside pattern, i.e. GM3 and GD1a content was decreased to about one-third compared with mock-transfected cells. At the same time, a 35{\%} increase in ganglioside GM1 content was observed. Mixed culture of MmNEU3-transfected cells with [1-3H]sphingosine-labeled cells demonstrates that the enzyme present at the cell surface is able to recognize gangliosides exposed on the membrane of nearby cells. Under these experimental conditions, the extent of ganglioside pattern changes was a function of MmNEU3 transient expression. Overall, the variations in GM3, GD1a, and GM1 content were very similar to those observed in the case of [1-3H]sphingosine-labeled MmNEU3-transfected cells, indicating that the enzyme mainly exerted its activity toward ganglioside substrates present at the surface of neighboring cells. These results indicate that the plasma membrane-associated sialidase MmNEU3 is able to hydrolyze ganglioside substrates in intact living cells at a neutral pH, mainly through cell-to-cell interactions.",
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AU - Croci, Gianluigi

AU - Bresciani, Roberto

AU - Yamaguchi, Kazunori

AU - Miyagi, Taeko

AU - Preti, Augusto

AU - Prinetti, Alessandro

AU - Prioni, Simona

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