The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines

Chiara Zurzolo, Raffaele Gentile, Anna Mascia, Corrado Garbi, Claudio Polistina, Luigi Aloj, Vittorio Enrico Avvedimento, Lucio Nitsch

Research output: Contribution to journalArticle

Abstract

We have studied the expression of cell polarity in hybrids between two rat thyroid epithelial cells: FRT and FRTL-5. FRT cells are polarized but do not express tissue-specific properties, FRTL-5 are unpolarized and express many thyroid-specific genes. A pool of 170 hybrid clones and five independent clones were characterized. The chromosome complement was that expected from 1:1 fusion of the parental cells. No chromosome loss was observed for several generations. All hybrids were polarized as judged from: (1) morphology, (2) transepithelial resistance, (3) preferential secretion of several proteins either through the apical (e.g. thyroglobulin) or through the basolateral pole, and (4) basolateral trapping of iodide. On the other hand, the expression of thyroid-specific markers: thyroglobulin synthesis and secretion, trapping of iodide, thyrotropin-dependent growth and expression of specific membrane antigens, were greatly reduced or inhibited in the pool and in the isolated clones. We also found that reduction of thyroglobulin synthesis was correlated with the loss of activity of the trans-acting factor TgTF1. We conclude that cell polarity, a property of FRT cells, is dominant in the hybrids whereas thyroid differentiation is recessive.

Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalJournal of Cell Science
Volume98
Issue number1
Publication statusPublished - Jan 1991

Fingerprint

Thyroglobulin
Cell Polarity
Thyroid Gland
Clone Cells
Iodides
Phenotype
Cell Line
Chromosomes
Trans-Activators
Cell Fusion
Thyrotropin
Antigens
Membranes
Growth
Genes
Proteins

Keywords

  • Hybrids
  • Polarity
  • Thyroid

ASJC Scopus subject areas

  • Cell Biology

Cite this

Zurzolo, C., Gentile, R., Mascia, A., Garbi, C., Polistina, C., Aloj, L., ... Nitsch, L. (1991). The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines. Journal of Cell Science, 98(1), 65-73.

The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines. / Zurzolo, Chiara; Gentile, Raffaele; Mascia, Anna; Garbi, Corrado; Polistina, Claudio; Aloj, Luigi; Avvedimento, Vittorio Enrico; Nitsch, Lucio.

In: Journal of Cell Science, Vol. 98, No. 1, 01.1991, p. 65-73.

Research output: Contribution to journalArticle

Zurzolo, C, Gentile, R, Mascia, A, Garbi, C, Polistina, C, Aloj, L, Avvedimento, VE & Nitsch, L 1991, 'The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines', Journal of Cell Science, vol. 98, no. 1, pp. 65-73.
Zurzolo, Chiara ; Gentile, Raffaele ; Mascia, Anna ; Garbi, Corrado ; Polistina, Claudio ; Aloj, Luigi ; Avvedimento, Vittorio Enrico ; Nitsch, Lucio. / The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines. In: Journal of Cell Science. 1991 ; Vol. 98, No. 1. pp. 65-73.
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