Abstract
We have studied the expression of cell polarity in hybrids between two rat thyroid epithelial cells: FRT and FRTL-5. FRT cells are polarized but do not express tissue-specific properties, FRTL-5 are unpolarized and express many thyroid-specific genes. A pool of 170 hybrid clones and five independent clones were characterized. The chromosome complement was that expected from 1:1 fusion of the parental cells. No chromosome loss was observed for several generations. All hybrids were polarized as judged from: (1) morphology, (2) transepithelial resistance, (3) preferential secretion of several proteins either through the apical (e.g. thyroglobulin) or through the basolateral pole, and (4) basolateral trapping of iodide. On the other hand, the expression of thyroid-specific markers: thyroglobulin synthesis and secretion, trapping of iodide, thyrotropin-dependent growth and expression of specific membrane antigens, were greatly reduced or inhibited in the pool and in the isolated clones. We also found that reduction of thyroglobulin synthesis was correlated with the loss of activity of the trans-acting factor TgTF1. We conclude that cell polarity, a property of FRT cells, is dominant in the hybrids whereas thyroid differentiation is recessive.
| Original language | English |
|---|---|
| Pages (from-to) | 65-73 |
| Number of pages | 9 |
| Journal | Journal of Cell Science |
| Volume | 98 |
| Issue number | 1 |
| Publication status | Published - Jan 1991 |
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Keywords
- Hybrids
- Polarity
- Thyroid
ASJC Scopus subject areas
- Cell Biology
Cite this
The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines. / Zurzolo, Chiara; Gentile, Raffaele; Mascia, Anna; Garbi, Corrado; Polistina, Claudio; Aloj, Luigi; Avvedimento, Vittorio Enrico; Nitsch, Lucio.
In: Journal of Cell Science, Vol. 98, No. 1, 01.1991, p. 65-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines
AU - Zurzolo, Chiara
AU - Gentile, Raffaele
AU - Mascia, Anna
AU - Garbi, Corrado
AU - Polistina, Claudio
AU - Aloj, Luigi
AU - Avvedimento, Vittorio Enrico
AU - Nitsch, Lucio
PY - 1991/1
Y1 - 1991/1
N2 - We have studied the expression of cell polarity in hybrids between two rat thyroid epithelial cells: FRT and FRTL-5. FRT cells are polarized but do not express tissue-specific properties, FRTL-5 are unpolarized and express many thyroid-specific genes. A pool of 170 hybrid clones and five independent clones were characterized. The chromosome complement was that expected from 1:1 fusion of the parental cells. No chromosome loss was observed for several generations. All hybrids were polarized as judged from: (1) morphology, (2) transepithelial resistance, (3) preferential secretion of several proteins either through the apical (e.g. thyroglobulin) or through the basolateral pole, and (4) basolateral trapping of iodide. On the other hand, the expression of thyroid-specific markers: thyroglobulin synthesis and secretion, trapping of iodide, thyrotropin-dependent growth and expression of specific membrane antigens, were greatly reduced or inhibited in the pool and in the isolated clones. We also found that reduction of thyroglobulin synthesis was correlated with the loss of activity of the trans-acting factor TgTF1. We conclude that cell polarity, a property of FRT cells, is dominant in the hybrids whereas thyroid differentiation is recessive.
AB - We have studied the expression of cell polarity in hybrids between two rat thyroid epithelial cells: FRT and FRTL-5. FRT cells are polarized but do not express tissue-specific properties, FRTL-5 are unpolarized and express many thyroid-specific genes. A pool of 170 hybrid clones and five independent clones were characterized. The chromosome complement was that expected from 1:1 fusion of the parental cells. No chromosome loss was observed for several generations. All hybrids were polarized as judged from: (1) morphology, (2) transepithelial resistance, (3) preferential secretion of several proteins either through the apical (e.g. thyroglobulin) or through the basolateral pole, and (4) basolateral trapping of iodide. On the other hand, the expression of thyroid-specific markers: thyroglobulin synthesis and secretion, trapping of iodide, thyrotropin-dependent growth and expression of specific membrane antigens, were greatly reduced or inhibited in the pool and in the isolated clones. We also found that reduction of thyroglobulin synthesis was correlated with the loss of activity of the trans-acting factor TgTF1. We conclude that cell polarity, a property of FRT cells, is dominant in the hybrids whereas thyroid differentiation is recessive.
KW - Hybrids
KW - Polarity
KW - Thyroid
UR - http://www.scopus.com/inward/record.url?scp=0026011363&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026011363&partnerID=8YFLogxK
M3 - Article
C2 - 1711531
AN - SCOPUS:0026011363
VL - 98
SP - 65
EP - 73
JO - Journal of Cell Science
JF - Journal of Cell Science
SN - 0021-9533
IS - 1
ER -