The position of the germinal vesicle and the chromatin organization together provide a marker of the developmental competence of mouse antral oocytes

Michele Bellone, Maurizio Zuccotti, Carlo Alberto Redi, Silvia Garagna

Research output: Contribution to journalArticle

Abstract

Based on their chromatin organization, antral oocytes can be classified into two classes, namely surrounded nucleolus (SN, chromatin forms a ring around the nucleolus), and not surrounded nucleolus (NSN, chromatin has a diffuse pattern). Oocytes of both classes are capable of meiotic resumption, but while SN oocytes, following fertilization, develop to term, NSN oocytes never develop beyond the two-cell stage. A recent study has shown that the position of the germinal vesicle (GV) can be used as a morphological marker predictive of oocyte meiotic competence, i.e. oocytes with a central GV have a higher meiotic competence than oocytes with an eccentric GV. In the present study, we have associated both markers with the aim of identifying, with more accuracy, the oocytes' developmental competence. Following their isolation, antral oocytes were classified on the basis of both SN and NSN chromatin configuration and their GV position, matured to metaphase II and fertilized in vitro. We demonstrated that the position of the GV is a good marker to predict the oocytes' developmental competence, but only when associated with the observation of the chromatin organization.

Original languageEnglish
Pages (from-to)639-643
Number of pages5
JournalReproduction
Volume138
Issue number4
DOIs
Publication statusPublished - Oct 2009

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

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