The possible role of ChemR23/Chemerin axis in the recruitment of dendritic cells in lupus nephritis

Giuseppe De Palma, Giuseppe Castellano, Annalisa Del Prete, Silvano Sozzani, Nicoletta Fiore, Antonia Loverre, Marc Parmentier, Loreto Gesualdo, Giuseppe Grandaliano, Francesco P. Schena

Research output: Contribution to journalArticlepeer-review

Abstract

Dendritic cells (DCs) have a pivotal role in the autoimmune response of systemic lupus erythematosus. Plasmacytoid DCs infiltrate the kidney of patients with lupus nephritis, but factors regulating their recruitment to the kidney are unknown. Chemerin is the recently identified natural ligand of ChemR23, a receptor highly expressed by plasmacytoid DCs. We performed immunohistochemical and immunofluorescence analysis to study the ChemR23/Chemerin axis in renal biopsies from patients with lupus nephritis. We found ChemR23-positive DCs had infiltrated the kidney tubulointerstitium in patients with severe lupus nephritis. Chemerin association with tubular epithelial cells and renal lymphatic endothelial cells was found in patients with lupus nephritis but not in normal kidneys. Proximal tubular epithelial cells produced Chemerin in vitro, which was significantly down-modulated by added tumor necrosis factor (TNF)-α and interferon-γ as measured by quantitative PCR and enzyme-linked immunosorbent assay. Interestingly, TNF-α was capable of inducing a functionally active form of renal Chemerin, resulting in an efficient transendothelial migration of plasmacytoid DCs measured in transwell systems. Thus, the ChemR23/Chemerin axis may have a role in the recruitment of DCs within the kidney in patients affected by lupus nephritis.

Original languageEnglish
Pages (from-to)1228-1235
Number of pages8
JournalKidney International
Volume79
Issue number11
DOIs
Publication statusPublished - Jun 2011

Keywords

  • chemokine
  • chemokine receptor
  • lupus nephritis
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Nephrology

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