The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort

H. Ghaedi, M. Tabasinezhad, B. Alipoor, F. Shokri, A. Movafagh, R. Mirfakhraie, M. D. Omrani, A. Masotti

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort. Methods: In this case–control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) for genotyping. Results: We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53–0.97). Conclusion: Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.

Original languageEnglish
Pages (from-to)1187-1193
Number of pages7
JournalJournal of Endocrinological Investigation
Volume39
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Type 2 Diabetes Mellitus
Blood Pressure
Alleles
Restriction Fragment Length Polymorphisms
LDL Cholesterol
Fasting
Triglycerides
Genotype
Weights and Measures
Glucose
Polymerase Chain Reaction
Population

Keywords

  • hsa-mir-27a
  • MicroRNA
  • Single nucleotide polymorphism
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort. / Ghaedi, H.; Tabasinezhad, M.; Alipoor, B.; Shokri, F.; Movafagh, A.; Mirfakhraie, R.; Omrani, M. D.; Masotti, A.

In: Journal of Endocrinological Investigation, Vol. 39, No. 10, 01.10.2016, p. 1187-1193.

Research output: Contribution to journalArticle

Ghaedi, H. ; Tabasinezhad, M. ; Alipoor, B. ; Shokri, F. ; Movafagh, A. ; Mirfakhraie, R. ; Omrani, M. D. ; Masotti, A. / The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort. In: Journal of Endocrinological Investigation. 2016 ; Vol. 39, No. 10. pp. 1187-1193.
@article{4a5c80aa50734d238bb7c36b803d4dce,
title = "The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort",
abstract = "Purpose: The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort. Methods: In this case–control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) for genotyping. Results: We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 {\%} CI 0.53–0.97). Conclusion: Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.",
keywords = "hsa-mir-27a, MicroRNA, Single nucleotide polymorphism, Type 2 diabetes mellitus",
author = "H. Ghaedi and M. Tabasinezhad and B. Alipoor and F. Shokri and A. Movafagh and R. Mirfakhraie and Omrani, {M. D.} and A. Masotti",
year = "2016",
month = "10",
day = "1",
doi = "10.1007/s40618-016-0499-4",
language = "English",
volume = "39",
pages = "1187--1193",
journal = "Journal of Endocrinological Investigation",
issn = "0391-4097",
publisher = "Springer International Publishing",
number = "10",

}

TY - JOUR

T1 - The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort

AU - Ghaedi, H.

AU - Tabasinezhad, M.

AU - Alipoor, B.

AU - Shokri, F.

AU - Movafagh, A.

AU - Mirfakhraie, R.

AU - Omrani, M. D.

AU - Masotti, A.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Purpose: The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort. Methods: In this case–control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) for genotyping. Results: We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53–0.97). Conclusion: Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.

AB - Purpose: The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort. Methods: In this case–control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) for genotyping. Results: We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53–0.97). Conclusion: Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.

KW - hsa-mir-27a

KW - MicroRNA

KW - Single nucleotide polymorphism

KW - Type 2 diabetes mellitus

UR - http://www.scopus.com/inward/record.url?scp=84987876196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84987876196&partnerID=8YFLogxK

U2 - 10.1007/s40618-016-0499-4

DO - 10.1007/s40618-016-0499-4

M3 - Article

VL - 39

SP - 1187

EP - 1193

JO - Journal of Endocrinological Investigation

JF - Journal of Endocrinological Investigation

SN - 0391-4097

IS - 10

ER -