The preliminary transcript map of a human skeletal muscle

Alberto Pallavicini, Rosanna Zimbello, Natascia Tiso, Teresa Muraro, Luca Rampoldi, Stefania Bortoluzzi, Giorgio Valle, Gerolamo Lanfranchi, Gian A. Danieli

Research output: Contribution to journalArticle

Abstract

By sequencing 11,405 individual expressed sequence tags (ESTs) from a cDNA library of a human skeletal muscle, we identified 1945 individual transcripts, 725 of which showed no correspondence with known human genes. We report here the chromosomal localization of 267 of these, obtained radiation hybrid (RH) mapping. The map position additional 242 ESTs from the same library, corresponding to known human genes, is also reported. The resulting information provides a preliminary genomic transcriptional profile of a human muscle. Several genes occur in clusters on different chromosomes. Moreover, chromosomes 17, 19, 21 and X appear to be significantly rich in muscle ESTs. By analysing several collections of ESTs from different tissues, we observed significant deviations in the distribution of ESTs by chromosome in fetal heart, adult brain and adult retina, supporting the hypothesis that a non-random localization of genes expressed in specific tissues might not be uncommon. The selective concentration of expressed genes in some chromosomes and in specific chromosomal subregions in a given tissue might reflect the existence of batteries of genes under the same control mechanisms, regulating tissue-specific gene expression.

Original languageEnglish
Pages (from-to)1445-1450
Number of pages6
JournalHuman Molecular Genetics
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 1997

ASJC Scopus subject areas

  • Genetics

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    Pallavicini, A., Zimbello, R., Tiso, N., Muraro, T., Rampoldi, L., Bortoluzzi, S., Valle, G., Lanfranchi, G., & Danieli, G. A. (1997). The preliminary transcript map of a human skeletal muscle. Human Molecular Genetics, 6(9), 1445-1450. https://doi.org/10.1093/hmg/6.9.1445