The presence of functional mannose receptor on macrophages at the maternal-fetal interface

Gordana Laskarin, K. Cupurdija, V. Sotosek Tokmadzic, D. Dorcic, J. Dupor, K. Juretic, N. Strbo, T. Bogovic Crncic, F. Marchezi, P. Allavena, A. Mantovani, Lj Randic, D. Rukavina

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Background: The mannose receptor (MR) is involved in the initiation of the immune response and regulation of homeostasis during inflammation and tissue remodeling. Methods: Distribution, endocytosis and possible natural ligand tumor associated glycoprotein-72 (TAG-72) for the MR have been examined by immunohistology, immunocytochemistry and flow cytometry at the maternal-fetal interface, characterized by extensive tissue remodeling. Results: Contrary to disseminated distribution of the MR positive (MR+) cells in term placenta, the MR+ cells of early pregnancy decidua intimately surrounded glands and followed tissue distribution of CD14 positive cells. The mannose receptor was present on freshly isolated first trimester decidual mononuclear cells and distributed mostly on macrophages (77.08 ± 10.55%, mean ± SD). The expression of the MR on CD14 positive cells decreased following 18 h culture (P <0.01) and was accompanied by the reduction of fluorescein isothiocyanate (FITC)-dextran uptake. PAM-1 anti-MR antibody, mannan and TAG-72 reduced FITC-dextran uptake by decidual macrophages. Conclusions: These data indicate that the MR+ macrophages, surrounding early decidual glands, are able to internalize ligands for carbohydrate recognition domain of the receptor, including decidual secretory phase mucin TAG-72.

Original languageEnglish
Pages (from-to)1057-1066
Number of pages10
JournalHuman Reproduction
Volume20
Issue number4
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Macrophages
Mothers
Ligands
Mannans
mannose receptor
Decidua
Mucins
Tissue Distribution
First Pregnancy Trimester
Endocytosis
Placenta
Flow Cytometry
Homeostasis
Immunohistochemistry
Carbohydrates
Inflammation
Pregnancy
Antibodies
tumor-associated antigen 72

Keywords

  • Decidua
  • Macrophages
  • Mannose receptor
  • Term pregnancy
  • Tumor associated glycoprotein-72

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Laskarin, G., Cupurdija, K., Sotosek Tokmadzic, V., Dorcic, D., Dupor, J., Juretic, K., ... Rukavina, D. (2005). The presence of functional mannose receptor on macrophages at the maternal-fetal interface. Human Reproduction, 20(4), 1057-1066. https://doi.org/10.1093/humrep/deh740

The presence of functional mannose receptor on macrophages at the maternal-fetal interface. / Laskarin, Gordana; Cupurdija, K.; Sotosek Tokmadzic, V.; Dorcic, D.; Dupor, J.; Juretic, K.; Strbo, N.; Bogovic Crncic, T.; Marchezi, F.; Allavena, P.; Mantovani, A.; Randic, Lj; Rukavina, D.

In: Human Reproduction, Vol. 20, No. 4, 04.2005, p. 1057-1066.

Research output: Contribution to journalArticle

Laskarin, G, Cupurdija, K, Sotosek Tokmadzic, V, Dorcic, D, Dupor, J, Juretic, K, Strbo, N, Bogovic Crncic, T, Marchezi, F, Allavena, P, Mantovani, A, Randic, L & Rukavina, D 2005, 'The presence of functional mannose receptor on macrophages at the maternal-fetal interface', Human Reproduction, vol. 20, no. 4, pp. 1057-1066. https://doi.org/10.1093/humrep/deh740
Laskarin G, Cupurdija K, Sotosek Tokmadzic V, Dorcic D, Dupor J, Juretic K et al. The presence of functional mannose receptor on macrophages at the maternal-fetal interface. Human Reproduction. 2005 Apr;20(4):1057-1066. https://doi.org/10.1093/humrep/deh740
Laskarin, Gordana ; Cupurdija, K. ; Sotosek Tokmadzic, V. ; Dorcic, D. ; Dupor, J. ; Juretic, K. ; Strbo, N. ; Bogovic Crncic, T. ; Marchezi, F. ; Allavena, P. ; Mantovani, A. ; Randic, Lj ; Rukavina, D. / The presence of functional mannose receptor on macrophages at the maternal-fetal interface. In: Human Reproduction. 2005 ; Vol. 20, No. 4. pp. 1057-1066.
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abstract = "Background: The mannose receptor (MR) is involved in the initiation of the immune response and regulation of homeostasis during inflammation and tissue remodeling. Methods: Distribution, endocytosis and possible natural ligand tumor associated glycoprotein-72 (TAG-72) for the MR have been examined by immunohistology, immunocytochemistry and flow cytometry at the maternal-fetal interface, characterized by extensive tissue remodeling. Results: Contrary to disseminated distribution of the MR positive (MR+) cells in term placenta, the MR+ cells of early pregnancy decidua intimately surrounded glands and followed tissue distribution of CD14 positive cells. The mannose receptor was present on freshly isolated first trimester decidual mononuclear cells and distributed mostly on macrophages (77.08 ± 10.55{\%}, mean ± SD). The expression of the MR on CD14 positive cells decreased following 18 h culture (P <0.01) and was accompanied by the reduction of fluorescein isothiocyanate (FITC)-dextran uptake. PAM-1 anti-MR antibody, mannan and TAG-72 reduced FITC-dextran uptake by decidual macrophages. Conclusions: These data indicate that the MR+ macrophages, surrounding early decidual glands, are able to internalize ligands for carbohydrate recognition domain of the receptor, including decidual secretory phase mucin TAG-72.",
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