The presence of lymphoid-associated antigens in adult acute myeloid leukemia is devoid of prognostic relevance

F. Lauria, D. Raspadori, M. A. Ventura, D. Rondelli, N. Testoni, P. Tosi, M. Michieli, D. Damiani, M. R. Motta, S. Tura

Research output: Contribution to journalArticlepeer-review


The immunophenotype of 110 adult patients with diagnosis of acute myeloblastic leukemia (AML) was analyzed using a wide panel of monoclonal antibodies (mAbs). Leukemic blasts were tested by applying direct immunofluorescence analysis and dual-fluorescence staining, and two groups of patients were identified: 56/110 (51%) expressing only myeloid antigens (My/AML) and 54/110 (49%) expressing both myeloid and lymphoid antigens (Ly/AML), CD13 and CD33 were expressed in almost all FAB subtypes, whereas CD14, frequently expressed in M4 and M5 subtypes (70%), was rarely expressed in M0 + M1 cases (9%). On the contrary, CD34, expressed in 77% of M0 + M1 cases, was practically absent in M3 and M5 subtypes (6% and 7%, respectively). CD2 and CD7 antigens were found in 34% and 42% of patients respectively, whereas B cell-associated antigens, such as CD10 and CD19, were found in 31% and 18% of patients. Cytogenetic abnormalities characteristically present in AML patients were also analyzed and, except for t(8;21) which was found in both groups of patients, the other abnormalities were frequently found in cases coexpressing lymphoid-associated antigens. Finally, the complete remission (CR) rate, survival and event-free survival were analyzed according to the presence of lymphoid markers and also of some specific antigens such as CD7 and CD34. The only prognostic difference was represented by CD34+ patients who showed a reduction in the CR rate compared with CD34- patients (65% versus 82%) (p = 0.05) which became more evident when the mean intensity of fluorescence was considered. In conclusion, mAbs conjugated with fluorochromes and analyzed by more sophisticated cytometers allow the identification of a higher number of AML cases bearing lymphoid- associated antigens, but this phenotypic coexpression is not associated with biologically distinct forms of leukemia.

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalStem Cells
Issue number4
Publication statusPublished - 1995


  • AML surface antigens
  • CD34
  • CD7
  • CR
  • mAbs

ASJC Scopus subject areas

  • Cell Biology


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