The Pro12Ala polymorphism of peroxisome proliferator-activated receptor-γ2 gene is associated with plasma levels of soluble RAGE (Receptor for Advanced Glycation Endproducts) and the presence of peripheral arterial disease

Mariella Catalano, Adriano Cortelazzo, Roberto Santi, Laura Contino, Marta Demicheli, Yusuf Yilmaz, Michele Zorzetto, Ilaria Campo, Niccolò Lanati, Enzo Emanuele

Research output: Contribution to journalArticle

Abstract

Objectives: Recent evidences suggest that the activation of peroxisome proliferator-activated receptor (PPAR)-γ2, which plays an important role in vascular homeostasis, also regulates the expression of the Receptor for Advanced Glycation End products (RAGE). In turn, low levels of soluble RAGE (sRAGE) have recently emerged as a valuable biomarker of vascular inflammation. The potential alterations in sRAGE concentrations in peripheral arterial disease (PAD), however, have not been yet investigated. The aim of the present study was to clarify whether the Pro12Ala polymorphism of the PPAR-γ2 gene is related to plasma sRAGE levels and the presence of PAD in nondiabetic Italian individuals. Design and methods: A total of 201 patients with PAD and 201 PAD-free control subjects were investigated. Genotyping of the Pro12Ala polymorphism of the PPAR-γ2 gene was performed by means of PCR-RFLPs. Plasma sRAGE levels were determined by ELISA. Results: Subjects carrying at least one Ala12 allele of the PPAR-γ2 gene had lower sRAGE levels (all p values <0.001). The prevalence rate of the Ala12 allele was significantly higher in PAD patients (14.0%) than in controls (8.0%, p = 0.009). In multivariate logistic regression analysis after adjustment for potential confounders, the Ala12 allele was significantly and independently associated with the risk of PAD (OR = 1.57, 95% CI = 1.11-2.65, p = 0.021). Conclusions: Our data indicate that the Ala12 allele of the PPAR-γ2 gene is associated with lower levels of the soluble decoy receptor sRAGE and the presence of PAD.

Original languageEnglish
Pages (from-to)981-985
Number of pages5
JournalClinical Biochemistry
Volume41
Issue number12
DOIs
Publication statusPublished - Aug 2008

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Keywords

  • Genetics
  • Peripheral arterial disease
  • Peroxisome proliferator-activated receptor
  • Polymorphism
  • Receptor for advanced glycation end products

ASJC Scopus subject areas

  • Clinical Biochemistry

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