The product of the human AHI-1 (Abelson helper integration site) gene: Experimental in vitro data point to its involvement in tumor cell invasion

Research output: Contribution to journalArticle

Abstract

Introduction. Each of the steps involved in invasion of tumors requires specific molecular program in which the modulation of adhesive and migratory properties of disseminating cells plays an essential role. The improvement in the knowledge of these mechanisms can lead to discovery of new target candidates in drug development. In this study we focused attention on the product of the human AHI-1 (Abelson helper integration site) gene Jouberin (Jbn). Methods. In particular, we explore by in vitro invasion assay, AHI-1 knockdown and electron microscopy, if Jbn is involved in the signaling machinery that regulates tumor invasion. To this purpose tumor cells of different histological derivation (brain, breast, skin) were employed. Results. We found that Jbn expression correlates with the proliferation, invasive potential and invasion strategy of the tested tumor cells, and that its downregulation reduces their capability of migrating and invading the extracellular matrix. Conclusions. The results obtained in this study for the first time point to Jbn as a new candidate involved in the invasion process of tumor cells, and as potential molecular target in anticancer therapy.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalAnnali dell'Istituto Superiore di Sanita
Volume53
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Genes
Neoplasms
Adhesives
Extracellular Matrix
Electron Microscopy
Breast
Down-Regulation
In Vitro Techniques
Skin
Brain
Pharmaceutical Preparations
Therapeutics

Keywords

  • Cell signalling
  • Electron microscopy
  • Invasion
  • Jouberin
  • Tumor cells

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

@article{c4efba8f29d9405d81d043b7ef8b9911,
title = "The product of the human AHI-1 (Abelson helper integration site) gene: Experimental in vitro data point to its involvement in tumor cell invasion",
abstract = "Introduction. Each of the steps involved in invasion of tumors requires specific molecular program in which the modulation of adhesive and migratory properties of disseminating cells plays an essential role. The improvement in the knowledge of these mechanisms can lead to discovery of new target candidates in drug development. In this study we focused attention on the product of the human AHI-1 (Abelson helper integration site) gene Jouberin (Jbn). Methods. In particular, we explore by in vitro invasion assay, AHI-1 knockdown and electron microscopy, if Jbn is involved in the signaling machinery that regulates tumor invasion. To this purpose tumor cells of different histological derivation (brain, breast, skin) were employed. Results. We found that Jbn expression correlates with the proliferation, invasive potential and invasion strategy of the tested tumor cells, and that its downregulation reduces their capability of migrating and invading the extracellular matrix. Conclusions. The results obtained in this study for the first time point to Jbn as a new candidate involved in the invasion process of tumor cells, and as potential molecular target in anticancer therapy.",
keywords = "Cell signalling, Electron microscopy, Invasion, Jouberin, Tumor cells",
author = "Giuseppina Bozzuto and Agnese Molinari",
year = "2017",
month = "1",
day = "1",
doi = "10.4415/ANN_17_01_05",
language = "English",
volume = "53",
pages = "17--24",
journal = "Annali dell'Istituto Superiore di Sanita",
issn = "0021-2571",
publisher = "Istituto Superiore di Sanita",
number = "1",

}

TY - JOUR

T1 - The product of the human AHI-1 (Abelson helper integration site) gene

T2 - Experimental in vitro data point to its involvement in tumor cell invasion

AU - Bozzuto, Giuseppina

AU - Molinari, Agnese

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Introduction. Each of the steps involved in invasion of tumors requires specific molecular program in which the modulation of adhesive and migratory properties of disseminating cells plays an essential role. The improvement in the knowledge of these mechanisms can lead to discovery of new target candidates in drug development. In this study we focused attention on the product of the human AHI-1 (Abelson helper integration site) gene Jouberin (Jbn). Methods. In particular, we explore by in vitro invasion assay, AHI-1 knockdown and electron microscopy, if Jbn is involved in the signaling machinery that regulates tumor invasion. To this purpose tumor cells of different histological derivation (brain, breast, skin) were employed. Results. We found that Jbn expression correlates with the proliferation, invasive potential and invasion strategy of the tested tumor cells, and that its downregulation reduces their capability of migrating and invading the extracellular matrix. Conclusions. The results obtained in this study for the first time point to Jbn as a new candidate involved in the invasion process of tumor cells, and as potential molecular target in anticancer therapy.

AB - Introduction. Each of the steps involved in invasion of tumors requires specific molecular program in which the modulation of adhesive and migratory properties of disseminating cells plays an essential role. The improvement in the knowledge of these mechanisms can lead to discovery of new target candidates in drug development. In this study we focused attention on the product of the human AHI-1 (Abelson helper integration site) gene Jouberin (Jbn). Methods. In particular, we explore by in vitro invasion assay, AHI-1 knockdown and electron microscopy, if Jbn is involved in the signaling machinery that regulates tumor invasion. To this purpose tumor cells of different histological derivation (brain, breast, skin) were employed. Results. We found that Jbn expression correlates with the proliferation, invasive potential and invasion strategy of the tested tumor cells, and that its downregulation reduces their capability of migrating and invading the extracellular matrix. Conclusions. The results obtained in this study for the first time point to Jbn as a new candidate involved in the invasion process of tumor cells, and as potential molecular target in anticancer therapy.

KW - Cell signalling

KW - Electron microscopy

KW - Invasion

KW - Jouberin

KW - Tumor cells

UR - http://www.scopus.com/inward/record.url?scp=85016806097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016806097&partnerID=8YFLogxK

U2 - 10.4415/ANN_17_01_05

DO - 10.4415/ANN_17_01_05

M3 - Article

C2 - 28361800

AN - SCOPUS:85016806097

VL - 53

SP - 17

EP - 24

JO - Annali dell'Istituto Superiore di Sanita

JF - Annali dell'Istituto Superiore di Sanita

SN - 0021-2571

IS - 1

ER -