The production of chromosomal alterations by β-lapachone an activator of topoisomerase I

Francesca Degrassi, Rosella De Salvia, Libera Berghella

Research output: Contribution to journalArticle

Abstract

The frequencies of chromosomal aberrations and sister-chromatid exchanges (SCE) after exposure to β-lapachone, an activator of mammalian topoiomerase I, were studied in Chinese hamster cells. A dose-dependent increase in the frequencies of SCE was observed in continuous treatments with β-lapachone. Chromatid-type aberrations were obtained in cells exposed to β-lapachone for one cell cycle but also in cells exposed during the G2 phase of the cell cycle, with a marked induction of exchange-type aberrations for both treatment schedules. We therefore propose that activation of topoisomerase I by β-lapachone results in the production of chromosomal alterations. The cell cycle dependence of β-lapachone clastogenic effects strongly suggests a mechanism for the formation of chromosomal aberrations after this drug closely resembling the one observed for the topoisomerase I inhibitor, camptothecin.

Original languageEnglish
Pages (from-to)263-267
Number of pages5
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume288
Issue number2
DOIs
Publication statusPublished - 1993

Fingerprint

Type I DNA Topoisomerase
Cell Cycle
Sister Chromatid Exchange
Chromosome Aberrations
Topoisomerase I Inhibitors
Camptothecin
Chromatids
G2 Phase
Cricetulus
Appointments and Schedules
Pharmaceutical Preparations

Keywords

  • Chromosomal aberrations
  • Sister-chromatid exchanges
  • Topoisomerase I
  • β-Lapachone

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis

Cite this

The production of chromosomal alterations by β-lapachone an activator of topoisomerase I. / Degrassi, Francesca; De Salvia, Rosella; Berghella, Libera.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 288, No. 2, 1993, p. 263-267.

Research output: Contribution to journalArticle

Degrassi, Francesca ; De Salvia, Rosella ; Berghella, Libera. / The production of chromosomal alterations by β-lapachone an activator of topoisomerase I. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 1993 ; Vol. 288, No. 2. pp. 263-267.
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