The proline-rich peptide Bac7(1-35) reduces mortality from Salmonella typhimurium in a mouse model of infection

Monica Benincasa, Chiara Pelillo, Sonia Zorzet, Chiara Garrovo, Stefania Biffi, Renato Gennaro, Marco Scocchi

Research output: Contribution to journalArticle

Abstract

Background. Bac7 is a proline-rich peptide with a potent in vitro antimicrobial activity against Gram-negative bacteria. Here we investigated its activity in biological fluids and in vivo using a mouse model of S. typhimurium infection. Results. The efficacy of the active 1-35 fragment of Bac7 was assayed in serum and plasma, and its stability in biological fluids analyzed by Western blot and mass spectrometry. The ability of the peptide to protect mice against Salmonella was assayed in a typhoid fever model of infection by determination of survival rates and bacterial load in liver and spleen of infected animals. In addition, the peptide's biodistribution was evaluated by using time-domain optical imaging. Bac7(1-35) retained a substantial in vivo activity showing a very low toxicity. The peptide increased significantly the number of survivors and the mean survival times of treated mice reducing the bacterial load in their organs despite its rapid clearance. Conclusions. Our results provide a first indication for a potential development of Bac7-based drugs in the treatment of salmonellosis and, eventually, other Gram-negative infections. The in vivo activity for this peptide might be substantially enhanced by decreasing its excretion rate or modifying the treatment schedule.

Original languageEnglish
Article number178
JournalBMC Microbiology
Volume10
DOIs
Publication statusPublished - 2010

Fingerprint

Salmonella typhimurium
Proline
Peptides
Mortality
Infection
Bacterial Load
Salmonella Infections
Survival Rate
Typhoid Fever
Optical Imaging
Gram-Negative Bacteria
Salmonella
Survivors
Mass Spectrometry
Appointments and Schedules
Spleen
Western Blotting
Bac7(1-35) peptide
bactenecin
Liver

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Benincasa, M., Pelillo, C., Zorzet, S., Garrovo, C., Biffi, S., Gennaro, R., & Scocchi, M. (2010). The proline-rich peptide Bac7(1-35) reduces mortality from Salmonella typhimurium in a mouse model of infection. BMC Microbiology, 10, [178]. https://doi.org/10.1186/1471-2180-10-178

The proline-rich peptide Bac7(1-35) reduces mortality from Salmonella typhimurium in a mouse model of infection. / Benincasa, Monica; Pelillo, Chiara; Zorzet, Sonia; Garrovo, Chiara; Biffi, Stefania; Gennaro, Renato; Scocchi, Marco.

In: BMC Microbiology, Vol. 10, 178, 2010.

Research output: Contribution to journalArticle

Benincasa, Monica ; Pelillo, Chiara ; Zorzet, Sonia ; Garrovo, Chiara ; Biffi, Stefania ; Gennaro, Renato ; Scocchi, Marco. / The proline-rich peptide Bac7(1-35) reduces mortality from Salmonella typhimurium in a mouse model of infection. In: BMC Microbiology. 2010 ; Vol. 10.
@article{33e64f80e02e4895b8026565957536dc,
title = "The proline-rich peptide Bac7(1-35) reduces mortality from Salmonella typhimurium in a mouse model of infection",
abstract = "Background. Bac7 is a proline-rich peptide with a potent in vitro antimicrobial activity against Gram-negative bacteria. Here we investigated its activity in biological fluids and in vivo using a mouse model of S. typhimurium infection. Results. The efficacy of the active 1-35 fragment of Bac7 was assayed in serum and plasma, and its stability in biological fluids analyzed by Western blot and mass spectrometry. The ability of the peptide to protect mice against Salmonella was assayed in a typhoid fever model of infection by determination of survival rates and bacterial load in liver and spleen of infected animals. In addition, the peptide's biodistribution was evaluated by using time-domain optical imaging. Bac7(1-35) retained a substantial in vivo activity showing a very low toxicity. The peptide increased significantly the number of survivors and the mean survival times of treated mice reducing the bacterial load in their organs despite its rapid clearance. Conclusions. Our results provide a first indication for a potential development of Bac7-based drugs in the treatment of salmonellosis and, eventually, other Gram-negative infections. The in vivo activity for this peptide might be substantially enhanced by decreasing its excretion rate or modifying the treatment schedule.",
author = "Monica Benincasa and Chiara Pelillo and Sonia Zorzet and Chiara Garrovo and Stefania Biffi and Renato Gennaro and Marco Scocchi",
year = "2010",
doi = "10.1186/1471-2180-10-178",
language = "English",
volume = "10",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "BioMed Central",

}

TY - JOUR

T1 - The proline-rich peptide Bac7(1-35) reduces mortality from Salmonella typhimurium in a mouse model of infection

AU - Benincasa, Monica

AU - Pelillo, Chiara

AU - Zorzet, Sonia

AU - Garrovo, Chiara

AU - Biffi, Stefania

AU - Gennaro, Renato

AU - Scocchi, Marco

PY - 2010

Y1 - 2010

N2 - Background. Bac7 is a proline-rich peptide with a potent in vitro antimicrobial activity against Gram-negative bacteria. Here we investigated its activity in biological fluids and in vivo using a mouse model of S. typhimurium infection. Results. The efficacy of the active 1-35 fragment of Bac7 was assayed in serum and plasma, and its stability in biological fluids analyzed by Western blot and mass spectrometry. The ability of the peptide to protect mice against Salmonella was assayed in a typhoid fever model of infection by determination of survival rates and bacterial load in liver and spleen of infected animals. In addition, the peptide's biodistribution was evaluated by using time-domain optical imaging. Bac7(1-35) retained a substantial in vivo activity showing a very low toxicity. The peptide increased significantly the number of survivors and the mean survival times of treated mice reducing the bacterial load in their organs despite its rapid clearance. Conclusions. Our results provide a first indication for a potential development of Bac7-based drugs in the treatment of salmonellosis and, eventually, other Gram-negative infections. The in vivo activity for this peptide might be substantially enhanced by decreasing its excretion rate or modifying the treatment schedule.

AB - Background. Bac7 is a proline-rich peptide with a potent in vitro antimicrobial activity against Gram-negative bacteria. Here we investigated its activity in biological fluids and in vivo using a mouse model of S. typhimurium infection. Results. The efficacy of the active 1-35 fragment of Bac7 was assayed in serum and plasma, and its stability in biological fluids analyzed by Western blot and mass spectrometry. The ability of the peptide to protect mice against Salmonella was assayed in a typhoid fever model of infection by determination of survival rates and bacterial load in liver and spleen of infected animals. In addition, the peptide's biodistribution was evaluated by using time-domain optical imaging. Bac7(1-35) retained a substantial in vivo activity showing a very low toxicity. The peptide increased significantly the number of survivors and the mean survival times of treated mice reducing the bacterial load in their organs despite its rapid clearance. Conclusions. Our results provide a first indication for a potential development of Bac7-based drugs in the treatment of salmonellosis and, eventually, other Gram-negative infections. The in vivo activity for this peptide might be substantially enhanced by decreasing its excretion rate or modifying the treatment schedule.

UR - http://www.scopus.com/inward/record.url?scp=77953711044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953711044&partnerID=8YFLogxK

U2 - 10.1186/1471-2180-10-178

DO - 10.1186/1471-2180-10-178

M3 - Article

C2 - 20573188

AN - SCOPUS:77953711044

VL - 10

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

M1 - 178

ER -