The promoter-associated noncoding RNA PNCCCND1_B assembles a protein–RNA complex to regulate cyclin D1 transcription in Ewing sarcoma

Ramona Palombo, Paola Frisone, Marco Fidaleo, Neri Mercatelli, Claudio Sette, Maria Paola Paronetto

Research output: Contribution to journalArticle

Abstract

Most Ewing sarcomas are characterized by the in-frame chromosomal translocation t(11;22) generating the EWS–FLI1 oncogene. EWS–FLI1 protein interacts with the RNA helicase DHX9 and affects transcription and processing of genes involved in neoplastic transformation, including CCND1 (the cyclin D1 gene), which contributes to cell-cycle dysregulation in cancer. In this study, we found that CCND1 expression is significantly higher in patients with Ewing sarcoma compared with other sarcomas and that the pncCCND1_B RNA, a previously uncharacterized CCND1 promoter-associated noncoding (pnc) transcript, is expressed in Ewing sarcoma cells. PncCCND1_B interacted with the RNA-binding protein Sam68 and repressed CCND1 expression. Notably, knockdown of Sam68 affected pncCCND1_B subcellular localization and cyclin D1 expression. Pharmacologic impairment of DHX9/EWS–FLI1 interaction promoted RNA-dependent association of Sam68 with DHX9 and recruitment of Sam68 to the CCND1 promoter, thus repressing it. Conversely, mitogenic stimulation of Ewing sarcoma cells with IGF1 impaired Sam68/DHX9 interaction and positively regulated CCND1 expression. These studies uncover a fine-tuned modulation of the proto-oncogene CCND1 in Ewing sarcoma cells via alternative complexes formed by DHX9 with either EWS–FLI1 or pncCCND1_B-Sam68.

Original languageEnglish
Pages (from-to)3570-3582
Number of pages13
JournalCancer Research
Volume79
Issue number14
DOIs
Publication statusPublished - Jan 1 2019

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bcl-1 Genes
Untranslated RNA
Ewing's Sarcoma
Cyclin D1
Gene Expression
RNA
RNA Helicases
Genetic Translocation
RNA-Binding Proteins
Proto-Oncogenes
Oncogenes
Sarcoma
Cell Cycle

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

The promoter-associated noncoding RNA PNCCCND1_B assembles a protein–RNA complex to regulate cyclin D1 transcription in Ewing sarcoma. / Palombo, Ramona; Frisone, Paola; Fidaleo, Marco; Mercatelli, Neri; Sette, Claudio; Paronetto, Maria Paola.

In: Cancer Research, Vol. 79, No. 14, 01.01.2019, p. 3570-3582.

Research output: Contribution to journalArticle

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