Abstract
The proliferating cell nuclear antigen (PCNA) gene codes for a protein that is necessary for cellular DNA synthesis and cell cycle progression. A functional promoter has been identified in the 5′ flanking region of the human PCNA gene. An abbreviated promoter (from the capsite to the PvuII restriction site at -395) was found to be equally efficient in directing transcription from a linked reporter, whether placed in the correct or reverse orientation in respect to the coding sequence. The reporter used was a cDNA of human thymidine kinase (TK), and the bidirectionality of the promoter was demonstrated by its ability to confer the TK+ phenotype to TK-ts13 cells and by the amount of specific message in RNA blots. The PvuII promoter placed between two coding sequences (the TK cDNA and the bacterial gene for neoresistance) is capable of driving transcription simultaneously in both directions. Finally, in blots of RNA from human cells, two transcripts could be detected that hybridized to a sense riboprobe from the 5′ flanking region of the human PCNA gene. We conclude that the locus for the human PCNA gene contains a bidirectional promoter producing diverging transcripts.
| Original language | English |
|---|---|
| Pages (from-to) | 286-293 |
| Number of pages | 8 |
| Journal | Experimental Cell Research |
| Volume | 188 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1990 |
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- Cell Biology
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The promoter of the human proliferating cell nuclear antigen (PCNA) gene is bidirectional. / Rizzo, Maria Giulia; Ottavio, Luigi; Travali, Salvatore; Chang, Chung der; Kaminska, Bozena; Baserga, Renato.
In: Experimental Cell Research, Vol. 188, No. 2, 1990, p. 286-293.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The promoter of the human proliferating cell nuclear antigen (PCNA) gene is bidirectional
AU - Rizzo, Maria Giulia
AU - Ottavio, Luigi
AU - Travali, Salvatore
AU - Chang, Chung der
AU - Kaminska, Bozena
AU - Baserga, Renato
PY - 1990
Y1 - 1990
N2 - The proliferating cell nuclear antigen (PCNA) gene codes for a protein that is necessary for cellular DNA synthesis and cell cycle progression. A functional promoter has been identified in the 5′ flanking region of the human PCNA gene. An abbreviated promoter (from the capsite to the PvuII restriction site at -395) was found to be equally efficient in directing transcription from a linked reporter, whether placed in the correct or reverse orientation in respect to the coding sequence. The reporter used was a cDNA of human thymidine kinase (TK), and the bidirectionality of the promoter was demonstrated by its ability to confer the TK+ phenotype to TK-ts13 cells and by the amount of specific message in RNA blots. The PvuII promoter placed between two coding sequences (the TK cDNA and the bacterial gene for neoresistance) is capable of driving transcription simultaneously in both directions. Finally, in blots of RNA from human cells, two transcripts could be detected that hybridized to a sense riboprobe from the 5′ flanking region of the human PCNA gene. We conclude that the locus for the human PCNA gene contains a bidirectional promoter producing diverging transcripts.
AB - The proliferating cell nuclear antigen (PCNA) gene codes for a protein that is necessary for cellular DNA synthesis and cell cycle progression. A functional promoter has been identified in the 5′ flanking region of the human PCNA gene. An abbreviated promoter (from the capsite to the PvuII restriction site at -395) was found to be equally efficient in directing transcription from a linked reporter, whether placed in the correct or reverse orientation in respect to the coding sequence. The reporter used was a cDNA of human thymidine kinase (TK), and the bidirectionality of the promoter was demonstrated by its ability to confer the TK+ phenotype to TK-ts13 cells and by the amount of specific message in RNA blots. The PvuII promoter placed between two coding sequences (the TK cDNA and the bacterial gene for neoresistance) is capable of driving transcription simultaneously in both directions. Finally, in blots of RNA from human cells, two transcripts could be detected that hybridized to a sense riboprobe from the 5′ flanking region of the human PCNA gene. We conclude that the locus for the human PCNA gene contains a bidirectional promoter producing diverging transcripts.
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U2 - 10.1016/0014-4827(90)90172-7
DO - 10.1016/0014-4827(90)90172-7
M3 - Article
C2 - 1970785
AN - SCOPUS:0025340086
VL - 188
SP - 286
EP - 293
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -