TY - JOUR
T1 - The promoter of the Proliferating Cell Nuclear Antigen (PCNA) gene is active in serum-deprived cells
AU - Ottavio, Luigi
AU - Chang, Chung Der
AU - Rizzo, Maria Giulia
AU - Petralia, Salvatore
AU - Travali, Salvatore
AU - Baserga, Renato
PY - 1990/6/15
Y1 - 1990/6/15
N2 - mRNA levels for the Proliferating Cell Nuclear Antigen (PCNA) gene are growth regulated. PCNA promoters of different lengths were used to drive a linked reporter, the cDNA for human thymidine kinase (TK). After transfection into TK-ts13 cells, stable cell lines were obtained. Regardless of promoter length, in all cell lines the levels of TK mRNA were roughly similar in serum-deprived and serum stimulated cells, confirming, by an independent method, that the growth regulation of PCNA mRNA levels does not depend on the 5′ flanking sequence of the PCNA gene.
AB - mRNA levels for the Proliferating Cell Nuclear Antigen (PCNA) gene are growth regulated. PCNA promoters of different lengths were used to drive a linked reporter, the cDNA for human thymidine kinase (TK). After transfection into TK-ts13 cells, stable cell lines were obtained. Regardless of promoter length, in all cell lines the levels of TK mRNA were roughly similar in serum-deprived and serum stimulated cells, confirming, by an independent method, that the growth regulation of PCNA mRNA levels does not depend on the 5′ flanking sequence of the PCNA gene.
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U2 - 10.1016/0006-291X(90)90360-Y
DO - 10.1016/0006-291X(90)90360-Y
M3 - Article
C2 - 1694073
AN - SCOPUS:0025306913
VL - 169
SP - 509
EP - 516
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -