The promoter of the Proliferating Cell Nuclear Antigen (PCNA) gene is active in serum-deprived cells

Luigi Ottavio, Chung Der Chang, Maria Giulia Rizzo, Salvatore Petralia, Salvatore Travali, Renato Baserga

Research output: Contribution to journalArticlepeer-review

Abstract

mRNA levels for the Proliferating Cell Nuclear Antigen (PCNA) gene are growth regulated. PCNA promoters of different lengths were used to drive a linked reporter, the cDNA for human thymidine kinase (TK). After transfection into TK-ts13 cells, stable cell lines were obtained. Regardless of promoter length, in all cell lines the levels of TK mRNA were roughly similar in serum-deprived and serum stimulated cells, confirming, by an independent method, that the growth regulation of PCNA mRNA levels does not depend on the 5′ flanking sequence of the PCNA gene.

Original languageEnglish
Pages (from-to)509-516
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume169
Issue number2
DOIs
Publication statusPublished - Jun 15 1990

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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