The properties of a tRNA-specific adenosine deaminase from Drosophila melanogaster support an evolutionary link between pre-mRNA editing and tRNA modification

Liam P. Keegan, André P. Gerber, Jim Brindle, Ronny Leemans, Angela Gallo, Walter Keller, Mary A. O'Connell

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Pre-mRNA editing involving the conversion of adenosine to inosine is mediated by adenosine deaminases that act on RNA (ADAR1 and ADAR2). ADARs contain multiple double-stranded RNA(dsRNA)-binding domains in addition to an adenosine deaminase domain. An adenosine deaminase acting on tRNAs, scTad1p (also known as scADAT1), cloned from Saccharomyces cerevisiae has a deaminase domain related to the ADARs but lacks dsRNA-binding domains. We have identified a gene homologous to scADAT1 in the region of Drosophila melanogaster Adh chromosome II. Recombinant Drosophila ADAT1 (dADAT1) has been expressed in the yeast Pichia pastoris and purified. The enzyme has no activity on dsRNA substrates but is a tRNA deaminase with specificity for adenosine 37 of insect alanine tRNA. dADAT1 shows greater similarity to vertebrate ADARs than to yeast Tad1p, supporting the hypothesis of a common evolutionary origin for ADARs and ADATs. dAdat1 transcripts are maternally supplied in the egg. Zygotic expression is widespread initially and later concentrates in the central nervous system.

Original languageEnglish
Pages (from-to)825-833
Number of pages9
JournalMolecular and Cellular Biology
Volume20
Issue number3
DOIs
Publication statusPublished - Feb 2000

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Adenosine Deaminase
RNA Precursors
Transfer RNA
Drosophila melanogaster
Adenosine
Drosophila
Yeasts
Inosine
Pichia
Double-Stranded RNA
Alanine
Ovum
Saccharomyces cerevisiae
Insects
Vertebrates
Central Nervous System
Chromosomes
RNA
Enzymes
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The properties of a tRNA-specific adenosine deaminase from Drosophila melanogaster support an evolutionary link between pre-mRNA editing and tRNA modification. / Keegan, Liam P.; Gerber, André P.; Brindle, Jim; Leemans, Ronny; Gallo, Angela; Keller, Walter; O'Connell, Mary A.

In: Molecular and Cellular Biology, Vol. 20, No. 3, 02.2000, p. 825-833.

Research output: Contribution to journalArticle

Keegan, Liam P. ; Gerber, André P. ; Brindle, Jim ; Leemans, Ronny ; Gallo, Angela ; Keller, Walter ; O'Connell, Mary A. / The properties of a tRNA-specific adenosine deaminase from Drosophila melanogaster support an evolutionary link between pre-mRNA editing and tRNA modification. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 3. pp. 825-833.
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