The protective role of catalase against cerebral ischemia in vitro and in vivo

M. Armogida, A. Spalloni, D. Amantea, M. Nutini, F. Petrelli, P. Longone, G. Bagetta, R. Nisticò, Nicola Biagio Mercuri

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The present study aims to assess the protective role of the antioxidant enzyme catalase (CAT) with relation to hydrogen peroxide (H 2O 2) degradation in oxygen plus water on electrophysiological and fluorescence changes induced by in vitro ischemia and on brain damage produced by transient in vivo ischemia. Neuroprotective effects of CAT were determined by means of electrophysiological recordings and confocal fluorescence microscopy in the hippocampal slice preparation. Ischemia was simulated in vitro by oxygen/glucose deprivation (OGD). In vivo ischemia was produced by transient middle cerebral artery occlusion (MCAo). A protection of the rat CA1 field excitatory postsynaptic potential (fEPSP) loss caused by a prolonged OGD (40 min) was observed after exogenous CAT (500 U/mL) bath-applied before a combined exposure to OGD and H 2O 2 (3 mM). Of note, neither H 2O 2 nor exogenous CAT alone had a protective action when OGD lasted for 40 min. The CAT-induced neuroprotection was confirmed in a transgenic mouse model over-expressing human CAT [Tg(CAT)]. In the presence of H 2O 2, the hippocampus of Tg(CAT) showed an increased resistance against OGD compared to that of wild-type (WT) animals. Moreover, CAT treatment reduced for about 50 min fEPSP depression evoked by repeated applications of H 2O 2 in normoxia. A lower sensitivity to H 2O 2-induced depression of fEPSPs was also indicated by the rightward shift of concentration-response curve in Tg(CAT) compared to WT mice. Noteworthy, Tg(CAT) mice had a reduced infarct size after MCAo. Our data suggest new strategies to reduce neuronal damage produced by transient brain ischemia through the manipulation of CAT enzyme.

Original languageEnglish
Pages (from-to)735-747
Number of pages13
JournalInternational Journal of Immunopathology and Pharmacology
Volume24
Issue number3
Publication statusPublished - Jul 2011

Fingerprint

Brain Ischemia
Catalase
Oxygen
Glucose
Ischemia
Excitatory Postsynaptic Potentials
Middle Cerebral Artery Infarction
In Vitro Techniques
Wild Animals
Neuroprotective Agents
Enzymes
Baths
Fluorescence Microscopy
Confocal Microscopy
Hydrogen Peroxide
Transgenic Mice
Hippocampus
Antioxidants
Fluorescence
Water

Keywords

  • Catalase
  • Cerebral ischemia
  • Neuroprotection
  • Rat
  • Transgenic mice

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

The protective role of catalase against cerebral ischemia in vitro and in vivo. / Armogida, M.; Spalloni, A.; Amantea, D.; Nutini, M.; Petrelli, F.; Longone, P.; Bagetta, G.; Nisticò, R.; Mercuri, Nicola Biagio.

In: International Journal of Immunopathology and Pharmacology, Vol. 24, No. 3, 07.2011, p. 735-747.

Research output: Contribution to journalArticle

Armogida, M. ; Spalloni, A. ; Amantea, D. ; Nutini, M. ; Petrelli, F. ; Longone, P. ; Bagetta, G. ; Nisticò, R. ; Mercuri, Nicola Biagio. / The protective role of catalase against cerebral ischemia in vitro and in vivo. In: International Journal of Immunopathology and Pharmacology. 2011 ; Vol. 24, No. 3. pp. 735-747.
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