The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

Claudio Procaccini; Fortunata Carbone; Dario Di Silvestre; Francesca Brambilla; Veronica De Rosa; Mario Galgani; Deriggio Faicchia; Gianni Marone; Donatella Tramontano; Marco Corona; Carlo Alviggi; Antonio Porcellini; Antonio La Cava; Pierluigi Mauri; Giuseppe Matarese

doi:10.1016/j.immuni.2016.01.028

The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

Claudio Procaccini, Fortunata Carbone, Dario Di Silvestre, Francesca Brambilla, Veronica De Rosa, Mario Galgani, Deriggio Faicchia, Gianni Marone, Donatella Tramontano, Marco Corona, Carlo Alviggi, Antonio Porcellini, Antonio La Cava, Pierluigi Mauri, Giuseppe Matarese

Research output: Contribution to journal › Article › peer-review

Abstract

Human CD4⁺CD25^hiFoxp3⁺CD127^- Treg and CD4⁺CD25^-Foxp3^- Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human Treg and Tconv cells that comprises analyses of the proteomic networks in subcellular compartments. We identified a dominant proteomic signature at the metabolic level that primarily impacted the highly-tuned balance between glucose and fatty-acid oxidation in the two cell types. Ex vivo Treg cells were highly glycolytic while Tconv cells used predominantly fatty-acid oxidation (FAO). When cultured in vitro, Treg cells engaged both glycolysis and FAO to proliferate, while Tconv cell proliferation mainly relied on glucose metabolism. Our unbiased proteomic analysis provides a molecular picture of the impact of metabolism on ex vivo human Treg versus Tconv cell functions that might be relevant for therapeutic manipulations of these cells.

Original language	English
Pages (from-to)	406-421
Number of pages	16
Journal	Immunity
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/j.immuni.2016.01.028
Publication status	Published - Feb 16 2016

Keywords

Conventional T cells
Immune Tolerance
Metabolism
Proteomic Analysis
Regulatory T cells

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases
Immunology

Access to Document

10.1016/j.immuni.2016.01.028

Cite this

Procaccini, C., Carbone, F., Di Silvestre, D., Brambilla, F., De Rosa, V., Galgani, M., Faicchia, D., Marone, G., Tramontano, D., Corona, M., Alviggi, C., Porcellini, A., La Cava, A., Mauri, P., & Matarese, G. (2016). The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. Immunity, 44(2), 406-421. https://doi.org/10.1016/j.immuni.2016.01.028

The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. / Procaccini, Claudio; Carbone, Fortunata; Di Silvestre, Dario; Brambilla, Francesca; De Rosa, Veronica; Galgani, Mario; Faicchia, Deriggio; Marone, Gianni; Tramontano, Donatella; Corona, Marco; Alviggi, Carlo; Porcellini, Antonio; La Cava, Antonio; Mauri, Pierluigi; Matarese, Giuseppe.

In: Immunity, Vol. 44, No. 2, 16.02.2016, p. 406-421.

Research output: Contribution to journal › Article › peer-review

Procaccini, C, Carbone, F, Di Silvestre, D, Brambilla, F, De Rosa, V, Galgani, M, Faicchia, D, Marone, G, Tramontano, D, Corona, M, Alviggi, C, Porcellini, A, La Cava, A, Mauri, P & Matarese, G 2016, 'The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements', Immunity, vol. 44, no. 2, pp. 406-421. https://doi.org/10.1016/j.immuni.2016.01.028

Procaccini, Claudio ; Carbone, Fortunata ; Di Silvestre, Dario ; Brambilla, Francesca ; De Rosa, Veronica ; Galgani, Mario ; Faicchia, Deriggio ; Marone, Gianni ; Tramontano, Donatella ; Corona, Marco ; Alviggi, Carlo ; Porcellini, Antonio ; La Cava, Antonio ; Mauri, Pierluigi ; Matarese, Giuseppe. / The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. In: Immunity. 2016 ; Vol. 44, No. 2. pp. 406-421.

@article{29b944fe8c6947c1b6681ad28f53db26,

title = "The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements",

abstract = "Human CD4+CD25hiFoxp3+CD127- Treg and CD4+CD25-Foxp3- Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human Treg and Tconv cells that comprises analyses of the proteomic networks in subcellular compartments. We identified a dominant proteomic signature at the metabolic level that primarily impacted the highly-tuned balance between glucose and fatty-acid oxidation in the two cell types. Ex vivo Treg cells were highly glycolytic while Tconv cells used predominantly fatty-acid oxidation (FAO). When cultured in vitro, Treg cells engaged both glycolysis and FAO to proliferate, while Tconv cell proliferation mainly relied on glucose metabolism. Our unbiased proteomic analysis provides a molecular picture of the impact of metabolism on ex vivo human Treg versus Tconv cell functions that might be relevant for therapeutic manipulations of these cells.",

keywords = "Conventional T cells, Immune Tolerance, Metabolism, Proteomic Analysis, Regulatory T cells",

author = "Claudio Procaccini and Fortunata Carbone and {Di Silvestre}, Dario and Francesca Brambilla and {De Rosa}, Veronica and Mario Galgani and Deriggio Faicchia and Gianni Marone and Donatella Tramontano and Marco Corona and Carlo Alviggi and Antonio Porcellini and {La Cava}, Antonio and Pierluigi Mauri and Giuseppe Matarese",

year = "2016",

month = feb,

day = "16",

doi = "10.1016/j.immuni.2016.01.028",

language = "English",

volume = "44",

pages = "406--421",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

AU - Procaccini, Claudio

AU - Carbone, Fortunata

AU - Di Silvestre, Dario

AU - Brambilla, Francesca

AU - De Rosa, Veronica

AU - Galgani, Mario

AU - Faicchia, Deriggio

AU - Marone, Gianni

AU - Tramontano, Donatella

AU - Corona, Marco

AU - Alviggi, Carlo

AU - Porcellini, Antonio

AU - La Cava, Antonio

AU - Mauri, Pierluigi

AU - Matarese, Giuseppe

PY - 2016/2/16

Y1 - 2016/2/16

N2 - Human CD4+CD25hiFoxp3+CD127- Treg and CD4+CD25-Foxp3- Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human Treg and Tconv cells that comprises analyses of the proteomic networks in subcellular compartments. We identified a dominant proteomic signature at the metabolic level that primarily impacted the highly-tuned balance between glucose and fatty-acid oxidation in the two cell types. Ex vivo Treg cells were highly glycolytic while Tconv cells used predominantly fatty-acid oxidation (FAO). When cultured in vitro, Treg cells engaged both glycolysis and FAO to proliferate, while Tconv cell proliferation mainly relied on glucose metabolism. Our unbiased proteomic analysis provides a molecular picture of the impact of metabolism on ex vivo human Treg versus Tconv cell functions that might be relevant for therapeutic manipulations of these cells.

AB - Human CD4+CD25hiFoxp3+CD127- Treg and CD4+CD25-Foxp3- Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human Treg and Tconv cells that comprises analyses of the proteomic networks in subcellular compartments. We identified a dominant proteomic signature at the metabolic level that primarily impacted the highly-tuned balance between glucose and fatty-acid oxidation in the two cell types. Ex vivo Treg cells were highly glycolytic while Tconv cells used predominantly fatty-acid oxidation (FAO). When cultured in vitro, Treg cells engaged both glycolysis and FAO to proliferate, while Tconv cell proliferation mainly relied on glucose metabolism. Our unbiased proteomic analysis provides a molecular picture of the impact of metabolism on ex vivo human Treg versus Tconv cell functions that might be relevant for therapeutic manipulations of these cells.

KW - Conventional T cells

KW - Immune Tolerance

KW - Metabolism

KW - Proteomic Analysis

KW - Regulatory T cells

UR - http://www.scopus.com/inward/record.url?scp=84958624127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958624127&partnerID=8YFLogxK

U2 - 10.1016/j.immuni.2016.01.028

DO - 10.1016/j.immuni.2016.01.028

M3 - Article

AN - SCOPUS:84958624127

VL - 44

SP - 406

EP - 421

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 2

ER -

The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this