Abstract
Human CD4+CD25hiFoxp3+CD127- Treg and CD4+CD25-Foxp3- Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human Treg and Tconv cells that comprises analyses of the proteomic networks in subcellular compartments. We identified a dominant proteomic signature at the metabolic level that primarily impacted the highly-tuned balance between glucose and fatty-acid oxidation in the two cell types. Ex vivo Treg cells were highly glycolytic while Tconv cells used predominantly fatty-acid oxidation (FAO). When cultured in vitro, Treg cells engaged both glycolysis and FAO to proliferate, while Tconv cell proliferation mainly relied on glucose metabolism. Our unbiased proteomic analysis provides a molecular picture of the impact of metabolism on ex vivo human Treg versus Tconv cell functions that might be relevant for therapeutic manipulations of these cells.
Original language | English |
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Pages (from-to) | 406-421 |
Number of pages | 16 |
Journal | Immunity |
Volume | 44 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 16 2016 |
Keywords
- Conventional T cells
- Immune Tolerance
- Metabolism
- Proteomic Analysis
- Regulatory T cells
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
- Immunology