The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

Claudio Procaccini; Fortunata Carbone; Dario Di Silvestre; Francesca Brambilla; Veronica De Rosa; Mario Galgani; Deriggio Faicchia; Gianni Marone; Donatella Tramontano; Marco Corona; Carlo Alviggi; Antonio Porcellini; Antonio La Cava; Pierluigi Mauri; Giuseppe Matarese

doi:10.1016/j.immuni.2016.01.028

The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

Claudio Procaccini, Fortunata Carbone, Dario Di Silvestre, Francesca Brambilla, Veronica De Rosa, Mario Galgani, Deriggio Faicchia, Gianni Marone, Donatella Tramontano, Marco Corona, Carlo Alviggi, Antonio Porcellini, Antonio La Cava, Pierluigi Mauri, Giuseppe Matarese

Research output: Contribution to journal › Article › peer-review

Abstract

Human CD4⁺CD25^hiFoxp3⁺CD127^- Treg and CD4⁺CD25^-Foxp3^- Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human Treg and Tconv cells that comprises analyses of the proteomic networks in subcellular compartments. We identified a dominant proteomic signature at the metabolic level that primarily impacted the highly-tuned balance between glucose and fatty-acid oxidation in the two cell types. Ex vivo Treg cells were highly glycolytic while Tconv cells used predominantly fatty-acid oxidation (FAO). When cultured in vitro, Treg cells engaged both glycolysis and FAO to proliferate, while Tconv cell proliferation mainly relied on glucose metabolism. Our unbiased proteomic analysis provides a molecular picture of the impact of metabolism on ex vivo human Treg versus Tconv cell functions that might be relevant for therapeutic manipulations of these cells.

Original language	English
Pages (from-to)	406-421
Number of pages	16
Journal	Immunity
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/j.immuni.2016.01.028
Publication status	Published - Feb 16 2016

Keywords

Conventional T cells
Immune Tolerance
Metabolism
Proteomic Analysis
Regulatory T cells

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases
Immunology

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10.1016/j.immuni.2016.01.028

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Procaccini, C., Carbone, F., Di Silvestre, D., Brambilla, F., De Rosa, V., Galgani, M., Faicchia, D., Marone, G., Tramontano, D., Corona, M., Alviggi, C., Porcellini, A., La Cava, A., Mauri, P., & Matarese, G. (2016). The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. Immunity, 44(2), 406-421. https://doi.org/10.1016/j.immuni.2016.01.028

Procaccini, C, Carbone, F, Di Silvestre, D, Brambilla, F, De Rosa, V, Galgani, M, Faicchia, D, Marone, G, Tramontano, D, Corona, M, Alviggi, C, Porcellini, A, La Cava, A, Mauri, P & Matarese, G 2016, 'The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements', Immunity, vol. 44, no. 2, pp. 406-421. https://doi.org/10.1016/j.immuni.2016.01.028

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AU - Alviggi, Carlo

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The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements

Abstract

Keywords

ASJC Scopus subject areas

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