TY - JOUR
T1 - The RB-related gene Rb2/p130 in neuroblastoma dierentiation and in B-myb promoter down-regulation
AU - Raschellà, Giuseppe
AU - Tanno, Barbara
AU - Bonetto, Francesco
AU - Negroni, Anna
AU - Claudio, Pier Paolo
AU - Baldi, Alfonso
AU - Amendola, Roberto
AU - Calabretta, Bruno
AU - Giordano, Antonio
AU - Paggi, Marco G.
PY - 1998/5
Y1 - 1998/5
N2 - The retinoblastoma family of nuclear factors is composed of RB, the prototype of the tumour suppressor genes and of the strictly related genes p107 and Rb2/p130. The three genes code for proteins, namely pRb, p107 and pRb2/p130, that share similar structures and functions. These proteins are expressed, often simultaneously, in many cell types and are involved in the regulation of proliferation and differentiation. We determined the expression and the phosphorylation of the RB family gene products during the DMSO-induced differentiation of the N1E-115 murine neuroblastoma cells. In this system, pRb2/p130 was strongly up-regulated during mid-late differentiation stages, while, on the contrary, pRb and p107 resulted markedly decreased at late stages. Differentiating N1E-115 cells also showed a progressive decrease in B-myb levels, a proliferation-related protein whose constitutive expression inhibits neuronal differentiation. Transfection of each of the RB family genes in these cells was able, at different degrees, to induce neuronal differentiation, to inhibit [3H]thymidine incorporation and to down-regulate the activity of the B-myb promoter.
AB - The retinoblastoma family of nuclear factors is composed of RB, the prototype of the tumour suppressor genes and of the strictly related genes p107 and Rb2/p130. The three genes code for proteins, namely pRb, p107 and pRb2/p130, that share similar structures and functions. These proteins are expressed, often simultaneously, in many cell types and are involved in the regulation of proliferation and differentiation. We determined the expression and the phosphorylation of the RB family gene products during the DMSO-induced differentiation of the N1E-115 murine neuroblastoma cells. In this system, pRb2/p130 was strongly up-regulated during mid-late differentiation stages, while, on the contrary, pRb and p107 resulted markedly decreased at late stages. Differentiating N1E-115 cells also showed a progressive decrease in B-myb levels, a proliferation-related protein whose constitutive expression inhibits neuronal differentiation. Transfection of each of the RB family genes in these cells was able, at different degrees, to induce neuronal differentiation, to inhibit [3H]thymidine incorporation and to down-regulate the activity of the B-myb promoter.
KW - Differentiation B-myb
KW - E2F
KW - Neuroblastoma
KW - Rb2/p130
KW - Retinoblastoma family
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UR - http://www.scopus.com/inward/citedby.url?scp=7344254104&partnerID=8YFLogxK
M3 - Article
C2 - 10200489
AN - SCOPUS:7344254104
VL - 5
SP - 401
EP - 407
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
SN - 1350-9047
IS - 5
ER -