The receptor encoded by the human c-MET oncogene is expressed in hepatocytes, epithelial cells and solid tumors

M. Prat, R. P. Narsimhan, T. Crepaldi, M. R. Nicotra, P. G. Natali, P. M. Comoglio

Research output: Contribution to journalArticle

273 Citations (Scopus)

Abstract

The human c-MET oncogene encodes a transmembrane tyrosine kinase (p190(c-met)) with structural and functional features of a growth-factor receptor. Monoclonal antibodies (MAbs) have been used to investigate the distribution of the c-Met protein in human normal and neoplastic tissues. By immunofluorescence microscopy homogeneous expression was detected in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Positive staining was also found in epithelial cells of the endometrium and ovary, and in basal keratinocytes of esophagus and skin. By Northern blot analysis, high levels of c-met messenger RNA were detected in specimens of liver, gastro-intestinal tract and kidney. c-met-specific mRNA was also found in thyroid, pancreas and placenta, in which organs c-Met protein was barely detectable by immunofluorescence. The antibodies revealed expression of c-MET protein in hepatomas (11/14), carcinomas of colon and rectum (19/21), stomach (11/22), kidney (16/19), ovary (9/17) and skin (7/17). Carcinomas of the lung (13/20), thyroid (11/13) and pancreas (5/7) were also positive. In these last cases (lung, thyroid and pancreas) tumor cells were homogeneously stained by the antibodies, whereas in their normal counterparts staining was barely detectable. These data suggest that the receptor encoded by c-MET plays a physiological role in epithelial cell growth and that its expression is altered in human carcinomas.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalInternational Journal of Cancer
Volume49
Issue number3
DOIs
Publication statusPublished - 1991

Fingerprint

Oncogenes
Proto-Oncogene Proteins c-met
Pancreas
Hepatocytes
Thyroid Gland
Epithelial Cells
Carcinoma
Ovary
Stomach
Staining and Labeling
Kidney
Neoplasms
Lung
Messenger RNA
Skin
Growth Factor Receptors
Antibodies
Large Intestine
Endometrium
Keratinocytes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The receptor encoded by the human c-MET oncogene is expressed in hepatocytes, epithelial cells and solid tumors. / Prat, M.; Narsimhan, R. P.; Crepaldi, T.; Nicotra, M. R.; Natali, P. G.; Comoglio, P. M.

In: International Journal of Cancer, Vol. 49, No. 3, 1991, p. 323-328.

Research output: Contribution to journalArticle

Prat, M. ; Narsimhan, R. P. ; Crepaldi, T. ; Nicotra, M. R. ; Natali, P. G. ; Comoglio, P. M. / The receptor encoded by the human c-MET oncogene is expressed in hepatocytes, epithelial cells and solid tumors. In: International Journal of Cancer. 1991 ; Vol. 49, No. 3. pp. 323-328.
@article{17438d7739d246aaaad2dc680703475e,
title = "The receptor encoded by the human c-MET oncogene is expressed in hepatocytes, epithelial cells and solid tumors",
abstract = "The human c-MET oncogene encodes a transmembrane tyrosine kinase (p190(c-met)) with structural and functional features of a growth-factor receptor. Monoclonal antibodies (MAbs) have been used to investigate the distribution of the c-Met protein in human normal and neoplastic tissues. By immunofluorescence microscopy homogeneous expression was detected in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Positive staining was also found in epithelial cells of the endometrium and ovary, and in basal keratinocytes of esophagus and skin. By Northern blot analysis, high levels of c-met messenger RNA were detected in specimens of liver, gastro-intestinal tract and kidney. c-met-specific mRNA was also found in thyroid, pancreas and placenta, in which organs c-Met protein was barely detectable by immunofluorescence. The antibodies revealed expression of c-MET protein in hepatomas (11/14), carcinomas of colon and rectum (19/21), stomach (11/22), kidney (16/19), ovary (9/17) and skin (7/17). Carcinomas of the lung (13/20), thyroid (11/13) and pancreas (5/7) were also positive. In these last cases (lung, thyroid and pancreas) tumor cells were homogeneously stained by the antibodies, whereas in their normal counterparts staining was barely detectable. These data suggest that the receptor encoded by c-MET plays a physiological role in epithelial cell growth and that its expression is altered in human carcinomas.",
author = "M. Prat and Narsimhan, {R. P.} and T. Crepaldi and Nicotra, {M. R.} and Natali, {P. G.} and Comoglio, {P. M.}",
year = "1991",
doi = "10.1002/ijc.2910490302",
language = "English",
volume = "49",
pages = "323--328",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - The receptor encoded by the human c-MET oncogene is expressed in hepatocytes, epithelial cells and solid tumors

AU - Prat, M.

AU - Narsimhan, R. P.

AU - Crepaldi, T.

AU - Nicotra, M. R.

AU - Natali, P. G.

AU - Comoglio, P. M.

PY - 1991

Y1 - 1991

N2 - The human c-MET oncogene encodes a transmembrane tyrosine kinase (p190(c-met)) with structural and functional features of a growth-factor receptor. Monoclonal antibodies (MAbs) have been used to investigate the distribution of the c-Met protein in human normal and neoplastic tissues. By immunofluorescence microscopy homogeneous expression was detected in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Positive staining was also found in epithelial cells of the endometrium and ovary, and in basal keratinocytes of esophagus and skin. By Northern blot analysis, high levels of c-met messenger RNA were detected in specimens of liver, gastro-intestinal tract and kidney. c-met-specific mRNA was also found in thyroid, pancreas and placenta, in which organs c-Met protein was barely detectable by immunofluorescence. The antibodies revealed expression of c-MET protein in hepatomas (11/14), carcinomas of colon and rectum (19/21), stomach (11/22), kidney (16/19), ovary (9/17) and skin (7/17). Carcinomas of the lung (13/20), thyroid (11/13) and pancreas (5/7) were also positive. In these last cases (lung, thyroid and pancreas) tumor cells were homogeneously stained by the antibodies, whereas in their normal counterparts staining was barely detectable. These data suggest that the receptor encoded by c-MET plays a physiological role in epithelial cell growth and that its expression is altered in human carcinomas.

AB - The human c-MET oncogene encodes a transmembrane tyrosine kinase (p190(c-met)) with structural and functional features of a growth-factor receptor. Monoclonal antibodies (MAbs) have been used to investigate the distribution of the c-Met protein in human normal and neoplastic tissues. By immunofluorescence microscopy homogeneous expression was detected in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Positive staining was also found in epithelial cells of the endometrium and ovary, and in basal keratinocytes of esophagus and skin. By Northern blot analysis, high levels of c-met messenger RNA were detected in specimens of liver, gastro-intestinal tract and kidney. c-met-specific mRNA was also found in thyroid, pancreas and placenta, in which organs c-Met protein was barely detectable by immunofluorescence. The antibodies revealed expression of c-MET protein in hepatomas (11/14), carcinomas of colon and rectum (19/21), stomach (11/22), kidney (16/19), ovary (9/17) and skin (7/17). Carcinomas of the lung (13/20), thyroid (11/13) and pancreas (5/7) were also positive. In these last cases (lung, thyroid and pancreas) tumor cells were homogeneously stained by the antibodies, whereas in their normal counterparts staining was barely detectable. These data suggest that the receptor encoded by c-MET plays a physiological role in epithelial cell growth and that its expression is altered in human carcinomas.

UR - http://www.scopus.com/inward/record.url?scp=0025954574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025954574&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910490302

DO - 10.1002/ijc.2910490302

M3 - Article

C2 - 1917129

AN - SCOPUS:0025954574

VL - 49

SP - 323

EP - 328

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 3

ER -