The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis

T. Kostalova, T. Lestinova, C. Maia, P. Sumova, M. Vlkova, L. Willen, N. Polanska, E. Fiorentino, A. Scalone, G. Oliva, F. Veronesi, J. M. Cristóvão, O. Courtenay, L. Campino, L. Gradoni, M. Gramiccia, P. Volf

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The frequency of sandfly–host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (SGH); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. Phlebotomus perniciosus (Diptera: Psychodidae) is the main vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in southwest Europe and is widespread from Portugal to Italy. In this study, sera were sampled from naturally exposed dogs from distant regions, including Campania (southern Italy), Umbria (central Italy) and the metropolitan Lisbon region (Portugal), where P. perniciosus is the unique or principal vector species. Sera were screened for anti-P. perniciosus antibodies using SGH and 43-kDa yellow-related recombinant protein (rSP03B). A robust correlation between antibodies recognizing SGH and rSP03B was detected in all regions, suggesting substantial antigenic cross-reactivity among different P. perniciosus populations. No significant differences in this relationship were detected between regions. Moreover, rSP03B and the native yellow-related protein were shown to share similar antigenic epitopes, as canine immunoglobulin G (IgG) binding to the native protein was inhibited by pre-incubation with the recombinant form. These findings suggest that rSP03B should be regarded as a universal marker of sandfly exposure throughout the geographical distribution of P. perniciosus.

Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalMedical and Veterinary Entomology
Volume31
Issue number1
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Phlebotomus perniciosus
leishmaniasis
Phlebotomus
Leishmaniasis
antigen
Recombinant Proteins
recombinant proteins
Psychodidae
Canidae
Salivary Glands
Italy
antibody
Dogs
Salivary Proteins and Peptides
screening
antigens
Antigens
salivary glands
protein
Portugal

Keywords

  • antibody response
  • dog
  • Leishmania infantum
  • markers of exposure
  • Mediterranean region
  • Phlebotomus spp.
  • salivary proteins
  • sandflies

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics
  • veterinary(all)
  • Insect Science

Cite this

The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis. / Kostalova, T.; Lestinova, T.; Maia, C.; Sumova, P.; Vlkova, M.; Willen, L.; Polanska, N.; Fiorentino, E.; Scalone, A.; Oliva, G.; Veronesi, F.; Cristóvão, J. M.; Courtenay, O.; Campino, L.; Gradoni, L.; Gramiccia, M.; Volf, P.

In: Medical and Veterinary Entomology, Vol. 31, No. 1, 01.03.2017, p. 88-93.

Research output: Contribution to journalArticle

Kostalova, T, Lestinova, T, Maia, C, Sumova, P, Vlkova, M, Willen, L, Polanska, N, Fiorentino, E, Scalone, A, Oliva, G, Veronesi, F, Cristóvão, JM, Courtenay, O, Campino, L, Gradoni, L, Gramiccia, M & Volf, P 2017, 'The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis', Medical and Veterinary Entomology, vol. 31, no. 1, pp. 88-93. https://doi.org/10.1111/mve.12192
Kostalova, T. ; Lestinova, T. ; Maia, C. ; Sumova, P. ; Vlkova, M. ; Willen, L. ; Polanska, N. ; Fiorentino, E. ; Scalone, A. ; Oliva, G. ; Veronesi, F. ; Cristóvão, J. M. ; Courtenay, O. ; Campino, L. ; Gradoni, L. ; Gramiccia, M. ; Volf, P. / The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis. In: Medical and Veterinary Entomology. 2017 ; Vol. 31, No. 1. pp. 88-93.
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abstract = "The frequency of sandfly–host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (SGH); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. Phlebotomus perniciosus (Diptera: Psychodidae) is the main vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in southwest Europe and is widespread from Portugal to Italy. In this study, sera were sampled from naturally exposed dogs from distant regions, including Campania (southern Italy), Umbria (central Italy) and the metropolitan Lisbon region (Portugal), where P. perniciosus is the unique or principal vector species. Sera were screened for anti-P. perniciosus antibodies using SGH and 43-kDa yellow-related recombinant protein (rSP03B). A robust correlation between antibodies recognizing SGH and rSP03B was detected in all regions, suggesting substantial antigenic cross-reactivity among different P. perniciosus populations. No significant differences in this relationship were detected between regions. Moreover, rSP03B and the native yellow-related protein were shown to share similar antigenic epitopes, as canine immunoglobulin G (IgG) binding to the native protein was inhibited by pre-incubation with the recombinant form. These findings suggest that rSP03B should be regarded as a universal marker of sandfly exposure throughout the geographical distribution of P. perniciosus.",
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