The relationship between chromosomal origins of replication and the nuclear matrix during the cell cycle

Maria Teresa Carrì, Gioacchino Micheli, Ermelinda Graziano, Tomasino Pace, Mario Buongiorno-Nardelli

Research output: Contribution to journalArticlepeer-review

Abstract

A cytological investigation into the dynamic behaviour of the origins of replication with respect to the nuclear matrix has been carried out on Xenopus laevis cultured cells. In order to preferentially label origins or 'non-origin' regions along DNA fibres, 5-fluoro-2′-deoxyuridine (FUdR)-treated cells were pulsed with [3H]deoxyadenosine in early or late S phase. Samples were then allowed to proceed through the cell cycle for increasing times. The DNA loops were induced in situ to completely uncoil around the nuclear matrix. The autoradiographic analysis shows that, under the experimental conditions used, 'non-origin' regions behave as expected from previous studies, i.e., they associate with the nuclear matrix only when they become part of a replication fork, whereas active origins of replication remain associated with the matrix throughout the cell cycle.

Original languageEnglish
Pages (from-to)426-436
Number of pages11
JournalExperimental Cell Research
Volume164
Issue number2
DOIs
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Cell Biology

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