The relevance of carbon dioxide metabolism in Streptococcus thermophilus

Stefania Arioli, Paola Roncada, Anna Maria Salzano, Francesca Deriu, Silvia Corona, Simone Guglielmetti, Luigi Bonizzi, Andrea Scaloni, Diego Mora

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Streptococcus thermophilus is a major component of dairy starter cultures used for the manufacture of yoghurt and cheese. In this study, the CO2 metabolism of S. thermophilus DSM 20617T, grown in either a N2 atmosphere or an enriched CO2 atmosphere, was analysed using both genetic and proteomic approaches. Growth experiments performed in a chemically defined medium revealed that CO2 depletion resulted in bacterial arginine, aspartate and uracil auxotrophy. Moreover, CO2 depletion governed a significant change in cell morphology, and a high reduction in biomass production. A comparative proteomic analysis revealed that cells of S. thermophilus showed a different degree of energy status depending on the CO2 availability. In agreement with proteomic data, cells grown under N2 showed a significantly higher milk acidification rate compared with those grown in an enriched CO2 atmosphere. Experiments carried out on S. thermophilus wild-type and its derivative mutant, which was inactivated in the phosphoenolpyruvate carboxylase and carbamoyl-phosphate synthase activities responsible for fixing CO2 to organic molecules, suggested that the anaplerotic reactions governed by these enzymes have a central role in bacterial metabolism. Our results reveal the capnophilic nature of this micro-organism, underlining the essential role of CO2 in S. thermophilus physiology, and suggesting potential applications in dairy fermentation processes.

Original languageEnglish
Pages (from-to)1953-1965
Number of pages13
JournalMicrobiology
Volume155
Issue number6
DOIs
Publication statusPublished - 2009

Fingerprint

Streptococcus thermophilus
Carbon Dioxide
Atmosphere
Proteomics
Phosphoenolpyruvate Carboxylase
Carbamyl Phosphate
Yogurt
Uracil
Cheese
Biomass
Fermentation
Milk
Enzymes
Growth

ASJC Scopus subject areas

  • Microbiology

Cite this

Arioli, S., Roncada, P., Salzano, A. M., Deriu, F., Corona, S., Guglielmetti, S., ... Mora, D. (2009). The relevance of carbon dioxide metabolism in Streptococcus thermophilus. Microbiology, 155(6), 1953-1965. https://doi.org/10.1099/mic.0.024737-0

The relevance of carbon dioxide metabolism in Streptococcus thermophilus. / Arioli, Stefania; Roncada, Paola; Salzano, Anna Maria; Deriu, Francesca; Corona, Silvia; Guglielmetti, Simone; Bonizzi, Luigi; Scaloni, Andrea; Mora, Diego.

In: Microbiology, Vol. 155, No. 6, 2009, p. 1953-1965.

Research output: Contribution to journalArticle

Arioli, S, Roncada, P, Salzano, AM, Deriu, F, Corona, S, Guglielmetti, S, Bonizzi, L, Scaloni, A & Mora, D 2009, 'The relevance of carbon dioxide metabolism in Streptococcus thermophilus', Microbiology, vol. 155, no. 6, pp. 1953-1965. https://doi.org/10.1099/mic.0.024737-0
Arioli S, Roncada P, Salzano AM, Deriu F, Corona S, Guglielmetti S et al. The relevance of carbon dioxide metabolism in Streptococcus thermophilus. Microbiology. 2009;155(6):1953-1965. https://doi.org/10.1099/mic.0.024737-0
Arioli, Stefania ; Roncada, Paola ; Salzano, Anna Maria ; Deriu, Francesca ; Corona, Silvia ; Guglielmetti, Simone ; Bonizzi, Luigi ; Scaloni, Andrea ; Mora, Diego. / The relevance of carbon dioxide metabolism in Streptococcus thermophilus. In: Microbiology. 2009 ; Vol. 155, No. 6. pp. 1953-1965.
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