The Reproducibility of the Immunohistochemical PD-L1 Testing in Non-Small-Cell Lung Cancer: A Multicentric Italian Experience

Elena Vigliar, Umberto Malapelle, Francesca Bono, Nicola Fusco, Diego Cortinovis, Emanuele Valtorta, Alexiadis Spyridon, Manuela Bimbatti, Mario Zocchi, Chiara Piva, Gabriella Gaudioso, Antonino Iaccarino, Patrizia Morbini, Fabio Pagni

Research output: Contribution to journalArticle

Abstract

An important harmonization effort was produced by the scientific community to standardize both the preanalytical and interpretative phases of programmed death-ligand 1 (PD-L1) immunohistochemical (IHC) testing in non-small-cell lung cancer (NSCLC). This analysis is crucial for the selection of patients with advanced-stage tumors eligible for treatment with pembrolizumab and potentially with other anti-PD-1/PD-L1 checkpoint inhibitors. This multicentric retrospective study evaluated the reproducibility of PD-L1 testing in the Italian scenario both for closed and open platforms. In the evaluation of the well-known gold-standard combinations (Agilent 22C3 PharmDx on Dako Autostainer versus Roche's Ventana SP263 on BenchMark), the results confirmed the literature data and showed complete overlapping between the two methods. With regard to the performances by using open platforms, the combination of 22C3 with Dako Omnis or Benchmark obtained good results basically, while the 28,8 clone seemed to be associated with worse scores.
Original languageEnglish
Pages (from-to)6832909
Number of pages1
JournalBioMed Research International
Volume2019
DOIs
Publication statusPublished - Apr 14 2019

Keywords

  • Adult
  • Antibodies, Monoclonal, Humanized/therapeutic use
  • B7-H1 Antigen/*analysis/antagonists & inhibitors
  • Biomarkers, Tumor
  • Carcinoma, Non-Small-Cell Lung/*diagnosis/drug therapy/*pathology
  • Carcinoma, Squamous Cell/pathology
  • Humans
  • Immunohistochemistry
  • Italy
  • Lung/pathology
  • Lung Neoplasms/*diagnosis/*pathology
  • Macrophages/pathology
  • Programmed Cell Death 1 Receptor
  • Retrospective Studies

Fingerprint Dive into the research topics of 'The Reproducibility of the Immunohistochemical PD-L1 Testing in Non-Small-Cell Lung Cancer: A Multicentric Italian Experience'. Together they form a unique fingerprint.

  • Cite this

    Vigliar, E., Malapelle, U., Bono, F., Fusco, N., Cortinovis, D., Valtorta, E., Spyridon, A., Bimbatti, M., Zocchi, M., Piva, C., Gaudioso, G., Iaccarino, A., Morbini, P., & Pagni, F. (2019). The Reproducibility of the Immunohistochemical PD-L1 Testing in Non-Small-Cell Lung Cancer: A Multicentric Italian Experience. BioMed Research International, 2019, 6832909. https://doi.org/10.1155/2019/6832909