The responses of lymphocytes from Asian and Caucasian diabetic patients and non-diabetics to hydrogen peroxide and sodium nitrite in the Comet assay

Natalie Wyatt, Christine Kelly, Vincenzo Fontana, Domenico Franco Merlo, Donald Whitelaw, Diana Anderson

Research output: Contribution to journalArticlepeer-review

Abstract

Numerous factors may influence the incidence of diabetes in the population. The production of reactive oxygen species (ROS) is elevated in diabetes patients. Based on the reported involvement of reactive species and nitrate/nitrite in diabetes, this present study has examined in the alkaline Comet assay, the effect of different levels of NaNO2 in the presence of the oxygen radical generating agent, hydrogen peroxide (H2O2). Peripheral lymphocytes from diabetic and non-diabetic Caucasians and Asians of both sexes were studied in vitro. Endogenous factors (e.g., sex, age, body mass index-BMI) and exogenous factors (lifestyle factors e.g., smoking and drinking habits, diet) were taken into account. A preliminary study in two individuals showed that DNA damage remained constant over a wide dose range of NaNO2 (1-75 mM), but when H2O2 was added at a constant concentration of 50 μM per dose of NaNO2, there was an increase in DNA damage corresponding with the varying levels of NaNO2 investigated. This was also seen with the 44 individuals (non-diabetic, n = 24; type 1 diabetic, n = 11; type 2 diabetic, n = 9) investigated. NaNO2 was capable of inducing a significant level of DNA damage in lymphocytes (p <0.001), but only with the addition of H2O2. When levels of DNA damage were analysed in terms of the different variables there were few significant differences in damage between diabetic and non-diabetic subjects, or other sub-population groups, and no statistically significant differences in susceptibility were observed between subject covariates using regression techniques.

Original languageEnglish
Pages (from-to)154-164
Number of pages11
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume609
Issue number2
DOIs
Publication statusPublished - Oct 30 2006

Keywords

  • Comet assay
  • Diabetes
  • Drinking water
  • Hydrogen peroxide
  • Nitrate
  • Sodium nitrite

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Fingerprint Dive into the research topics of 'The responses of lymphocytes from Asian and Caucasian diabetic patients and non-diabetics to hydrogen peroxide and sodium nitrite in the Comet assay'. Together they form a unique fingerprint.

Cite this