The retinoblastoma-related Rb2/p130 gene is an effector downstream of AP-2 during neural differentiation

Marco G. Paggi, Francesco Bonetto, Anna Severino, Alfonso Baldi, Tullio Battista, Federica Bucci, Armando Felsani, Daniela Lombardi, Antonio Giordano

Research output: Contribution to journalArticle

Abstract

Rb2/p130, a member of the Retinoblastoma family of growth and tumour suppressor genes, is extensively implicated in the control of cell cycle and differentiation. The minimal promoter region of Rb2/p130 in T98G human glioblastoma cells was identified and its analysis revealed the presence of a KER1 palindromic sequence able to bind the transcription factor AP-2, a regulatory protein that plays a crucial role in ectodermal differentiation. This KER1 site interacted in vitro with AP-2, and AP-2 overexpression increased Rb2/p130 transcription and translation. We also found that rat PC12 pheochromocytoma cells, when induced to differentiate by NGF, displayed an increase of AP-2 protein levels and of Rb2/p130 transcription and protein levels. AP-2-transfected PC12 cells displayed enhanced transcription and translation of Rb2/p130 and of the cdk inhibitor p21WAFI/CIPI, a gene known to be under the control of AP-2, but unable by itself to elicit PC12 differentiation. Overexpression of either AP-2 or Rb2/p130 elicited per se cell differentiation in the absence of NGF, while coexpression of AP-2B, a negative regulator of AP-2 transcriptional activity, inhibited only AP-2-induced differentiation. Altogether, these results indicate that Rb2/p130 is a critical effector of AP-2 in sustaining ectodermal differentiation.

Original languageEnglish
Pages (from-to)2570-2578
Number of pages9
JournalOncogene
Volume20
Issue number20
DOIs
Publication statusPublished - May 3 2001

Fingerprint

Retinoblastoma
PC12 Cells
Nerve Growth Factor
Tumor Suppressor Genes
Cell Differentiation
Transcription Factor AP-2
Genes
Proteins
Pheochromocytoma
Glioblastoma
Genetic Promoter Regions

Keywords

  • AP-2
  • Differentiation
  • NGF
  • PC12
  • Rb2/p130

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Paggi, M. G., Bonetto, F., Severino, A., Baldi, A., Battista, T., Bucci, F., ... Giordano, A. (2001). The retinoblastoma-related Rb2/p130 gene is an effector downstream of AP-2 during neural differentiation. Oncogene, 20(20), 2570-2578. https://doi.org/10.1038/sj.onc.1204356

The retinoblastoma-related Rb2/p130 gene is an effector downstream of AP-2 during neural differentiation. / Paggi, Marco G.; Bonetto, Francesco; Severino, Anna; Baldi, Alfonso; Battista, Tullio; Bucci, Federica; Felsani, Armando; Lombardi, Daniela; Giordano, Antonio.

In: Oncogene, Vol. 20, No. 20, 03.05.2001, p. 2570-2578.

Research output: Contribution to journalArticle

Paggi, MG, Bonetto, F, Severino, A, Baldi, A, Battista, T, Bucci, F, Felsani, A, Lombardi, D & Giordano, A 2001, 'The retinoblastoma-related Rb2/p130 gene is an effector downstream of AP-2 during neural differentiation', Oncogene, vol. 20, no. 20, pp. 2570-2578. https://doi.org/10.1038/sj.onc.1204356
Paggi, Marco G. ; Bonetto, Francesco ; Severino, Anna ; Baldi, Alfonso ; Battista, Tullio ; Bucci, Federica ; Felsani, Armando ; Lombardi, Daniela ; Giordano, Antonio. / The retinoblastoma-related Rb2/p130 gene is an effector downstream of AP-2 during neural differentiation. In: Oncogene. 2001 ; Vol. 20, No. 20. pp. 2570-2578.
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