The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA

Simona Segalla, Silvia Pivetti, Katia Todoerti, Malgorzata Agata Chudzik, Erica Claudia Giuliani, Federico Lazzaro, Viviana Volta, Dejan Lazarevic, Giovanna Musco, Marco Muzi-Falconi, Antonino Neri, Stefano Biffo, Giovanni Tonon

Research output: Contribution to journalArticle

Abstract

Multiple myeloma, the second most frequent hematologic tumor after lymphomas, is an incurable cancer. Recent sequencing efforts have identified the ribonuclease DIS3 as one of the most frequently mutated genes in this disease. DIS3 represents the catalytic subunit of the exosome, a macromolecular complex central to the processing, maturation and surveillance of various RNAs. miRNAs are an evolutionarily conserved class of small noncoding RNAs, regulating gene expression at post-transcriptional level. Ribonucleases, including Drosha, Dicer and XRN2, are involved in the processing and stability of miRNAs. However, the role of DIS3 on the regulation of miRNAs remains largely unknown. Here we found that DIS3 regulates the levels of the tumor suppressor let-7 miRNAs without affecting other miRNA families. DIS3 facilitates the maturation of let-7 miRNAs by reducing in the cytoplasm the RNA stability of the pluripotency factor LIN28B, a inhibitor of let-7 processing. DIS3 inactivation, through the increase of LIN28B and the reduction of mature let-7, enhances the translation of let-7 targets such as MYC and RAS leading to enhanced tumorigenesis. Our study establishes that the ribonuclease DIS3, targeting LIN28B, sustains the maturation of let-7 miRNAs and suggests the increased translation of critical oncogenes as one of the biological outcomes of DIS3 inactivation.

Original languageEnglish
Pages (from-to)5182-5193
Number of pages12
JournalNucleic Acids Research
Volume43
Issue number10
DOIs
Publication statusPublished - Apr 12 2015

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Ribonucleases
MicroRNAs
Messenger RNA
Exosomes
Macromolecular Substances
Small Untranslated RNA
Neoplasms
RNA Stability
Multiple Myeloma
Oncogenes
Lymphoma
Catalytic Domain
Carcinogenesis
Cytoplasm
RNA
Gene Expression
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Segalla, S., Pivetti, S., Todoerti, K., Chudzik, M. A., Giuliani, E. C., Lazzaro, F., ... Tonon, G. (2015). The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA. Nucleic Acids Research, 43(10), 5182-5193. https://doi.org/10.1093/nar/gkv387

The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA. / Segalla, Simona; Pivetti, Silvia; Todoerti, Katia; Chudzik, Malgorzata Agata; Giuliani, Erica Claudia; Lazzaro, Federico; Volta, Viviana; Lazarevic, Dejan; Musco, Giovanna; Muzi-Falconi, Marco; Neri, Antonino; Biffo, Stefano; Tonon, Giovanni.

In: Nucleic Acids Research, Vol. 43, No. 10, 12.04.2015, p. 5182-5193.

Research output: Contribution to journalArticle

Segalla, S, Pivetti, S, Todoerti, K, Chudzik, MA, Giuliani, EC, Lazzaro, F, Volta, V, Lazarevic, D, Musco, G, Muzi-Falconi, M, Neri, A, Biffo, S & Tonon, G 2015, 'The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA', Nucleic Acids Research, vol. 43, no. 10, pp. 5182-5193. https://doi.org/10.1093/nar/gkv387
Segalla S, Pivetti S, Todoerti K, Chudzik MA, Giuliani EC, Lazzaro F et al. The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA. Nucleic Acids Research. 2015 Apr 12;43(10):5182-5193. https://doi.org/10.1093/nar/gkv387
Segalla, Simona ; Pivetti, Silvia ; Todoerti, Katia ; Chudzik, Malgorzata Agata ; Giuliani, Erica Claudia ; Lazzaro, Federico ; Volta, Viviana ; Lazarevic, Dejan ; Musco, Giovanna ; Muzi-Falconi, Marco ; Neri, Antonino ; Biffo, Stefano ; Tonon, Giovanni. / The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA. In: Nucleic Acids Research. 2015 ; Vol. 43, No. 10. pp. 5182-5193.
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