The ribosomal P0 protein induces a spontaneous immune response in patients with head and neck advanced stage carcinoma that is not dependent on its overexpression in carcinomas

Roberto Bei, Laura Masuelli, Paola Trono, Pier Luigi Orvietani, Simona Losito, Laura Marzocchella, Domenico Vitolo, Loredana Albonici, Marie Agnes Mrozek, Elena Di Gennaro, Florigio Lista, Giovanni Faggioni, Franco Ionna, Luciano Binaglia, Vittorio Manzari, Alfredo Budillon, Andrea Modesti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A typical feature in systemic lupus erythemathosus patients is the presence of autoantibodies to the carboxylterminal homologous P proteins (P0, P1, P2) domain (C-22 P0 epitope). In this report we provide evidence for the in vivo immunogenicity of the P0 protein in head and neck cancer patients as well as overexpression by immunohistochemistry of the C-22 P0 epitope in invasive carcinomas (55/57). Overexpression of this epitope was also significantly associated with a number of pathological lesions arising in the head and neck stratified epithelium including acanthosis (8/8), benign tumors (11/11), dysplasia (23/25) and in situ carcinomas (9/9). Intermediate cell layer restricted epitope overexpression was observed in well differentiated carcinomas, while undifferentiated tumors showed overexpression throughout the cell layers. Employing recombinant P proteins, sera from 40 of the 57 carcinoma patients and 39 normal donors, were subjected to immunoblot analysis. Immunity to P0 protein (7/40) was associated with malignancy and with advanced disease stage, but it was not dependent on the C-22 P0 epitope overexpression, although it was the preferential autoantibody target in 4 patients. Increased expression of the C-22 P0 epitope on the surface of pharynx cancer cells following cellular stress in vitro, may imply P0 protein presentation as an in vivo autoantibody target in cancer patients. Evidence for immunity to the P0 protein, as well as overexpression in patients with head and neck carcinoma may be relevant for monitoring cancer progression, in planning immunotherapeutic strategies, and contribute to the understanding of immuno-biological behaviour of head and neck carcinomas.

Original languageEnglish
Pages (from-to)1301-1308
Number of pages8
JournalInternational Journal of Oncology
Volume31
Issue number6
Publication statusPublished - Dec 2007

Fingerprint

Myelin P0 Protein
Epitopes
Neck
Head
Carcinoma
Autoantibodies
Neoplasms
Immunity
Pharyngeal Neoplasms
Carcinoma in Situ
Head and Neck Neoplasms
Recombinant Proteins
ribosomal protein P0
Epithelium
Immunohistochemistry
Tissue Donors
Serum

Keywords

  • Cancer patients
  • Head and neck
  • Immune response
  • Ribosomal P0 protein
  • Tumor associated antigens

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The ribosomal P0 protein induces a spontaneous immune response in patients with head and neck advanced stage carcinoma that is not dependent on its overexpression in carcinomas. / Bei, Roberto; Masuelli, Laura; Trono, Paola; Orvietani, Pier Luigi; Losito, Simona; Marzocchella, Laura; Vitolo, Domenico; Albonici, Loredana; Mrozek, Marie Agnes; Di Gennaro, Elena; Lista, Florigio; Faggioni, Giovanni; Ionna, Franco; Binaglia, Luciano; Manzari, Vittorio; Budillon, Alfredo; Modesti, Andrea.

In: International Journal of Oncology, Vol. 31, No. 6, 12.2007, p. 1301-1308.

Research output: Contribution to journalArticle

Bei, R, Masuelli, L, Trono, P, Orvietani, PL, Losito, S, Marzocchella, L, Vitolo, D, Albonici, L, Mrozek, MA, Di Gennaro, E, Lista, F, Faggioni, G, Ionna, F, Binaglia, L, Manzari, V, Budillon, A & Modesti, A 2007, 'The ribosomal P0 protein induces a spontaneous immune response in patients with head and neck advanced stage carcinoma that is not dependent on its overexpression in carcinomas', International Journal of Oncology, vol. 31, no. 6, pp. 1301-1308.
Bei, Roberto ; Masuelli, Laura ; Trono, Paola ; Orvietani, Pier Luigi ; Losito, Simona ; Marzocchella, Laura ; Vitolo, Domenico ; Albonici, Loredana ; Mrozek, Marie Agnes ; Di Gennaro, Elena ; Lista, Florigio ; Faggioni, Giovanni ; Ionna, Franco ; Binaglia, Luciano ; Manzari, Vittorio ; Budillon, Alfredo ; Modesti, Andrea. / The ribosomal P0 protein induces a spontaneous immune response in patients with head and neck advanced stage carcinoma that is not dependent on its overexpression in carcinomas. In: International Journal of Oncology. 2007 ; Vol. 31, No. 6. pp. 1301-1308.
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abstract = "A typical feature in systemic lupus erythemathosus patients is the presence of autoantibodies to the carboxylterminal homologous P proteins (P0, P1, P2) domain (C-22 P0 epitope). In this report we provide evidence for the in vivo immunogenicity of the P0 protein in head and neck cancer patients as well as overexpression by immunohistochemistry of the C-22 P0 epitope in invasive carcinomas (55/57). Overexpression of this epitope was also significantly associated with a number of pathological lesions arising in the head and neck stratified epithelium including acanthosis (8/8), benign tumors (11/11), dysplasia (23/25) and in situ carcinomas (9/9). Intermediate cell layer restricted epitope overexpression was observed in well differentiated carcinomas, while undifferentiated tumors showed overexpression throughout the cell layers. Employing recombinant P proteins, sera from 40 of the 57 carcinoma patients and 39 normal donors, were subjected to immunoblot analysis. Immunity to P0 protein (7/40) was associated with malignancy and with advanced disease stage, but it was not dependent on the C-22 P0 epitope overexpression, although it was the preferential autoantibody target in 4 patients. Increased expression of the C-22 P0 epitope on the surface of pharynx cancer cells following cellular stress in vitro, may imply P0 protein presentation as an in vivo autoantibody target in cancer patients. Evidence for immunity to the P0 protein, as well as overexpression in patients with head and neck carcinoma may be relevant for monitoring cancer progression, in planning immunotherapeutic strategies, and contribute to the understanding of immuno-biological behaviour of head and neck carcinomas.",
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AU - Bei, Roberto

AU - Masuelli, Laura

AU - Trono, Paola

AU - Orvietani, Pier Luigi

AU - Losito, Simona

AU - Marzocchella, Laura

AU - Vitolo, Domenico

AU - Albonici, Loredana

AU - Mrozek, Marie Agnes

AU - Di Gennaro, Elena

AU - Lista, Florigio

AU - Faggioni, Giovanni

AU - Ionna, Franco

AU - Binaglia, Luciano

AU - Manzari, Vittorio

AU - Budillon, Alfredo

AU - Modesti, Andrea

PY - 2007/12

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N2 - A typical feature in systemic lupus erythemathosus patients is the presence of autoantibodies to the carboxylterminal homologous P proteins (P0, P1, P2) domain (C-22 P0 epitope). In this report we provide evidence for the in vivo immunogenicity of the P0 protein in head and neck cancer patients as well as overexpression by immunohistochemistry of the C-22 P0 epitope in invasive carcinomas (55/57). Overexpression of this epitope was also significantly associated with a number of pathological lesions arising in the head and neck stratified epithelium including acanthosis (8/8), benign tumors (11/11), dysplasia (23/25) and in situ carcinomas (9/9). Intermediate cell layer restricted epitope overexpression was observed in well differentiated carcinomas, while undifferentiated tumors showed overexpression throughout the cell layers. Employing recombinant P proteins, sera from 40 of the 57 carcinoma patients and 39 normal donors, were subjected to immunoblot analysis. Immunity to P0 protein (7/40) was associated with malignancy and with advanced disease stage, but it was not dependent on the C-22 P0 epitope overexpression, although it was the preferential autoantibody target in 4 patients. Increased expression of the C-22 P0 epitope on the surface of pharynx cancer cells following cellular stress in vitro, may imply P0 protein presentation as an in vivo autoantibody target in cancer patients. Evidence for immunity to the P0 protein, as well as overexpression in patients with head and neck carcinoma may be relevant for monitoring cancer progression, in planning immunotherapeutic strategies, and contribute to the understanding of immuno-biological behaviour of head and neck carcinomas.

AB - A typical feature in systemic lupus erythemathosus patients is the presence of autoantibodies to the carboxylterminal homologous P proteins (P0, P1, P2) domain (C-22 P0 epitope). In this report we provide evidence for the in vivo immunogenicity of the P0 protein in head and neck cancer patients as well as overexpression by immunohistochemistry of the C-22 P0 epitope in invasive carcinomas (55/57). Overexpression of this epitope was also significantly associated with a number of pathological lesions arising in the head and neck stratified epithelium including acanthosis (8/8), benign tumors (11/11), dysplasia (23/25) and in situ carcinomas (9/9). Intermediate cell layer restricted epitope overexpression was observed in well differentiated carcinomas, while undifferentiated tumors showed overexpression throughout the cell layers. Employing recombinant P proteins, sera from 40 of the 57 carcinoma patients and 39 normal donors, were subjected to immunoblot analysis. Immunity to P0 protein (7/40) was associated with malignancy and with advanced disease stage, but it was not dependent on the C-22 P0 epitope overexpression, although it was the preferential autoantibody target in 4 patients. Increased expression of the C-22 P0 epitope on the surface of pharynx cancer cells following cellular stress in vitro, may imply P0 protein presentation as an in vivo autoantibody target in cancer patients. Evidence for immunity to the P0 protein, as well as overexpression in patients with head and neck carcinoma may be relevant for monitoring cancer progression, in planning immunotherapeutic strategies, and contribute to the understanding of immuno-biological behaviour of head and neck carcinomas.

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