Abstract
The fusion of the β-galactosidase structural gene to a DNA fragment that contains the promoter of the primer for initiation of pMB1 replication in vitro has allowed the isolation of a mutation in this promoter. By recombination in vivo, this mutation was introduced into a plasmid that contains all the information essential for plasmid replication. Since the resulting mutant plasmid is not able to replicate, it is concluded that transcription starting from the promoter of the in vitro primer precursor is essential for pMB1 replication.
| Original language | English |
|---|---|
| Pages (from-to) | 123-126 |
| Number of pages | 4 |
| Journal | Journal of Molecular Biology |
| Volume | 160 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Sep 5 1982 |
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ASJC Scopus subject areas
- Virology
Cite this
The RNA primer promoter, as defined in vitro, is essential for pMB1 plasmid replication in vivo. / Cesareni, Gianni.
In: Journal of Molecular Biology, Vol. 160, No. 1, 05.09.1982, p. 123-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The RNA primer promoter, as defined in vitro, is essential for pMB1 plasmid replication in vivo
AU - Cesareni, Gianni
PY - 1982/9/5
Y1 - 1982/9/5
N2 - The fusion of the β-galactosidase structural gene to a DNA fragment that contains the promoter of the primer for initiation of pMB1 replication in vitro has allowed the isolation of a mutation in this promoter. By recombination in vivo, this mutation was introduced into a plasmid that contains all the information essential for plasmid replication. Since the resulting mutant plasmid is not able to replicate, it is concluded that transcription starting from the promoter of the in vitro primer precursor is essential for pMB1 replication.
AB - The fusion of the β-galactosidase structural gene to a DNA fragment that contains the promoter of the primer for initiation of pMB1 replication in vitro has allowed the isolation of a mutation in this promoter. By recombination in vivo, this mutation was introduced into a plasmid that contains all the information essential for plasmid replication. Since the resulting mutant plasmid is not able to replicate, it is concluded that transcription starting from the promoter of the in vitro primer precursor is essential for pMB1 replication.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020185066&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(82)90135-8
DO - 10.1016/0022-2836(82)90135-8
M3 - Article
C2 - 6816942
AN - SCOPUS:0020185066
VL - 160
SP - 123
EP - 126
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -