The role of 2 FOXP3 isoforms in the generation of human CD4+ Tregs

Sarah E. Allan, Laura Passerini, Rosa Bacchetta, Natasha Crellin, Minyue Dai, Paul C. Orban, Steven F. Ziegler, Maria Grazia Roncarolo, Megan K. Levings

Research output: Contribution to journalArticle

Abstract

Little is known about the molecules that control the development and function of CD4+CD25+ Tregs. Recently, it was shown that the transcription factor FOXP3 is necessary and sufficient for the generation of CD4+CD25+ Tregs in mice. We investigated the capacity of FOXP3 to drive the generation of suppressive CD4+CD25+ Tregs in humans. Surprisingly, although ectopic expression of FOXP3 in human CD4+ T cells resulted in induction of hyporesponsiveness and suppression of IL-2 production, it did not lead to acquisition of significant suppressor activity in vitro. Similarly, ectopic expression of FOXP3Δ2, an isoform found in human CD4+CD25+ Tregs that lacks exon 2, also failed to induce the development of suppressor T cells. Moreover, when FOXP3 and FOXP3Δ2 were simultaneously overexpressed, although the expression of several Treg-associated cell surface markers was significantly increased, only a modest suppressive activity was induced. These data indicate that in humans, overexpression of FOXP3 alone or together with FOXP3Δ2 is not an effective method to generate potent suppressor T cells in vitro and suggest that factors in addition to FOXP3 are required during the process of activation and/or differentiation for the development of bona fide Tregs.

Original languageEnglish
Pages (from-to)3276-3284
Number of pages9
JournalJournal of Clinical Investigation
Volume115
Issue number11
DOIs
Publication statusPublished - Nov 2005

ASJC Scopus subject areas

  • Medicine(all)

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    Allan, S. E., Passerini, L., Bacchetta, R., Crellin, N., Dai, M., Orban, P. C., Ziegler, S. F., Roncarolo, M. G., & Levings, M. K. (2005). The role of 2 FOXP3 isoforms in the generation of human CD4+ Tregs. Journal of Clinical Investigation, 115(11), 3276-3284. https://doi.org/10.1172/JCI24685