The role of cell adhesion in retinoic acid-induced modulation of chondrocyte phenotype

M. Sanchez, A. Arcella, Gianfranco Pontarelli, E. Gionti

Research output: Contribution to journalArticle

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Abstract

Retinoic acid (RA) treatment of a suspension of quail chondrocytes inhibits the expression of cartilage collagens and induces cell adhesion along with fibronectin expression. We asked whether the RA-induced modulation of the chondrocyte phenotype was dependent on cell adhesion. Prevention of cell adhesion blocks cell growth and many of the effects associated with RA, such as collagen II inhibition, collagen I activation and fibronectin induction. The activity of the bone/tendon promoter of the α2(I) collagen gene was determined by measuring the transient expression of COL1A2-CAT, a chimaeric gene bearing 3500 bp from upstream of the transcription start site of the human α2(I) gene fused to the chloramphenicol acetyltransferase (CAT) gene. This promoter is activated only in permissive conditions for cell adhesion. The attachment activities of chondrocytes on protein substrates was studied by an in vitro cell adhesion assay. Untreated cells or cells maintained in suspension while undergoing RA treatment do not attach when replated on protein substrates. Chondrocytes treated with RA in permissive conditions for cell adhesion rapidly attach and spread instead on collagen-coated wells. Altogether the results suggest that cell adhesion plays a major role in RA-induced modulation of the chondrocyte phenotype.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalBiochemical Journal
Volume313
Issue number1
Publication statusPublished - Jan 1 1996

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Cell adhesion
Chondrocytes
Tretinoin
Cell Adhesion
Modulation
Phenotype
Collagen
Genes
Chloramphenicol O-Acetyltransferase
Fibronectins
Suspensions
Bearings (structural)
Quail
Transcription Initiation Site
Tendons
Cartilage
Cell growth
Substrates
Assays
Bone

ASJC Scopus subject areas

  • Biochemistry

Cite this

The role of cell adhesion in retinoic acid-induced modulation of chondrocyte phenotype. / Sanchez, M.; Arcella, A.; Pontarelli, Gianfranco; Gionti, E.

In: Biochemical Journal, Vol. 313, No. 1, 01.01.1996, p. 201-206.

Research output: Contribution to journalArticle

Sanchez, M, Arcella, A, Pontarelli, G & Gionti, E 1996, 'The role of cell adhesion in retinoic acid-induced modulation of chondrocyte phenotype', Biochemical Journal, vol. 313, no. 1, pp. 201-206.
Sanchez, M. ; Arcella, A. ; Pontarelli, Gianfranco ; Gionti, E. / The role of cell adhesion in retinoic acid-induced modulation of chondrocyte phenotype. In: Biochemical Journal. 1996 ; Vol. 313, No. 1. pp. 201-206.
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