TY - JOUR
T1 - The role of CXCR3 and its ligands CXCL10 and CXCL11 in the pathogenesis of celiac disease
AU - Haghbin, Mahrokh
AU - Rostami-Nejad, Mohammad
AU - Forouzesh, Flora
AU - Sadeghi, Amir
AU - Rostami, Kamran
AU - Aghamohammadi, Elham
AU - Asadzadeh-Aghdaei, Hamid
AU - Masotti, Andrea
AU - Zali, Mohammad Reza
PY - 2019/6
Y1 - 2019/6
N2 - The chemokine receptor CXCR3 and its ligands CXCL10 and CXCL11 have been suggested to give rise to the most relevant chemokine axis able to facilitate the entrance of immune cells into inflamed tissues and be activated in different inflammatory disorders, such as celiac disease (CD).The aim of this study was to investigate the expression level of CXCR3, CXCL10, and CXCL11 genes in celiac patients compared to healthy controls. Both cohorts have been recruited from the Iranian population.In this case-control study, biopsy specimens were collected from 71 celiac patients (60.5% female) and 90 control subjects (57% female) during 2016. Total RNA was extracted and mRNA expression levels of CXCR3, CXCL10, and CXCL11 genes were investigated by SYBR green qPCR.Based on qPCR and relative quantification method, the mRNA expression levels of CXCR3, CXCL10, and CXCL11 were significantly higher in duodenal biopsies of celiac patients compared to healthy controls in the study population (P = .038, P = .021, and P = .012 respectively).The result of this study showed that CXCR3/CXCL10/CXCL11 signaling axis is overexpressed in the small intestinal mucosa of CD patients compared to controls. This finding might explain the specific enrollment of the main cell populations that infiltrate the epithelium.
AB - The chemokine receptor CXCR3 and its ligands CXCL10 and CXCL11 have been suggested to give rise to the most relevant chemokine axis able to facilitate the entrance of immune cells into inflamed tissues and be activated in different inflammatory disorders, such as celiac disease (CD).The aim of this study was to investigate the expression level of CXCR3, CXCL10, and CXCL11 genes in celiac patients compared to healthy controls. Both cohorts have been recruited from the Iranian population.In this case-control study, biopsy specimens were collected from 71 celiac patients (60.5% female) and 90 control subjects (57% female) during 2016. Total RNA was extracted and mRNA expression levels of CXCR3, CXCL10, and CXCL11 genes were investigated by SYBR green qPCR.Based on qPCR and relative quantification method, the mRNA expression levels of CXCR3, CXCL10, and CXCL11 were significantly higher in duodenal biopsies of celiac patients compared to healthy controls in the study population (P = .038, P = .021, and P = .012 respectively).The result of this study showed that CXCR3/CXCL10/CXCL11 signaling axis is overexpressed in the small intestinal mucosa of CD patients compared to controls. This finding might explain the specific enrollment of the main cell populations that infiltrate the epithelium.
KW - Adult
KW - Case-Control Studies
KW - Celiac Disease/genetics
KW - Chemokine CXCL10/genetics
KW - Chemokine CXCL11/genetics
KW - Cohort Studies
KW - European Continental Ancestry Group/genetics
KW - Female
KW - Gene Expression Regulation
KW - Humans
KW - Iran
KW - Male
KW - Receptors, CXCR3/genetics
U2 - 10.1097/MD.0000000000015949
DO - 10.1097/MD.0000000000015949
M3 - Article
C2 - 31232926
VL - 98
SP - e15949
JO - Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries
JF - Medicine; analytical reviews of general medicine, neurology, psychiatry, dermatology, and pediatries
SN - 0025-7974
IS - 25
ER -