The role of CXCR3 and its ligands CXCL10 and CXCL11 in the pathogenesis of celiac disease

Mahrokh Haghbin, Mohammad Rostami-Nejad, Flora Forouzesh, Amir Sadeghi, Kamran Rostami, Elham Aghamohammadi, Hamid Asadzadeh-Aghdaei, Andrea Masotti, Mohammad Reza Zali

Research output: Contribution to journalArticle

Abstract

The chemokine receptor CXCR3 and its ligands CXCL10 and CXCL11 have been suggested to give rise to the most relevant chemokine axis able to facilitate the entrance of immune cells into inflamed tissues and be activated in different inflammatory disorders, such as celiac disease (CD).The aim of this study was to investigate the expression level of CXCR3, CXCL10, and CXCL11 genes in celiac patients compared to healthy controls. Both cohorts have been recruited from the Iranian population.In this case-control study, biopsy specimens were collected from 71 celiac patients (60.5% female) and 90 control subjects (57% female) during 2016. Total RNA was extracted and mRNA expression levels of CXCR3, CXCL10, and CXCL11 genes were investigated by SYBR green qPCR.Based on qPCR and relative quantification method, the mRNA expression levels of CXCR3, CXCL10, and CXCL11 were significantly higher in duodenal biopsies of celiac patients compared to healthy controls in the study population (P = .038, P = .021, and P = .012 respectively).The result of this study showed that CXCR3/CXCL10/CXCL11 signaling axis is overexpressed in the small intestinal mucosa of CD patients compared to controls. This finding might explain the specific enrollment of the main cell populations that infiltrate the epithelium.

Original languageEnglish
Pages (from-to)e15949
JournalMedicine
Volume98
Issue number25
DOIs
Publication statusPublished - Jun 2019

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Keywords

  • Adult
  • Case-Control Studies
  • Celiac Disease/genetics
  • Chemokine CXCL10/genetics
  • Chemokine CXCL11/genetics
  • Cohort Studies
  • European Continental Ancestry Group/genetics
  • Female
  • Gene Expression Regulation
  • Humans
  • Iran
  • Male
  • Receptors, CXCR3/genetics

Cite this

Haghbin, M., Rostami-Nejad, M., Forouzesh, F., Sadeghi, A., Rostami, K., Aghamohammadi, E., Asadzadeh-Aghdaei, H., Masotti, A., & Zali, M. R. (2019). The role of CXCR3 and its ligands CXCL10 and CXCL11 in the pathogenesis of celiac disease. Medicine, 98(25), e15949. https://doi.org/10.1097/MD.0000000000015949